No evidence of a death-like function for species B1 human adenovirus type 3 E3-9K during A549 cell line infection

Kathryn M. Frietze, Samuel K Campos, Adriana E. Kajon

Research output: Contribution to journalArticle

Abstract

Background: Subspecies B1 human adenoviruses (HAdV-B1) are prevalent respiratory pathogens. Compared to their species C (HAdV-C) counterparts, relatively little work has been devoted to the characterization of their unique molecular biology. The early region 3 (E3) transcription unit is an interesting target for future efforts because of its species-specific diversity in genetic content among adenoviruses. This diversity is particularly significant for the subset of E3-encoded products that are membrane glycoproteins and may account for the distinct pathobiology of the different human adenovirus species. In order to understand the role of HAdV-B-specific genes in viral pathogenesis, we initiated the characterization of unique E3 genes. As a continuation of our efforts to define the function encoded in the highly polymorphic ORF E3-10.9K and testing the hypothesis that the E3-10.9K protein orthologs with a hydrophobic domain contribute to the efficient release of viral progeny, we generated HAdV-3 mutant viruses unable to express E3-10.9K ortholog E3-9K and examined their ability to grow, disseminate, and egress in cell culture. Results: No differences were observed in the kinetics of infected cell death, and virus progeny release or in the plaque size and dissemination phenotypes between cells infected with HAdV-3 E3-9K mutants or the parental virus. The ectopic expression of E3-10.9K orthologs with a hydrophobic domain did not compromise cell viability. Conclusions: Our data show that despite the remarkable similarities with HAdV-C E3-11.6K, HAdV-B1 ORF E3-10.9K does not encode a product with a death-like biological activity.

Original languageEnglish (US)
Article number429
JournalBMC Research Notes
Volume5
DOIs
StatePublished - 2012

Fingerprint

Human Adenoviruses
Viruses
Open Reading Frames
Cells
Virus Release
Viral Genes
Membrane Glycoproteins
Genes
Infection
Adenoviridae
Molecular Biology
Cell Survival
Molecular biology
Cell Death
Cell Culture Techniques
Pathogens
Cell death
Transcription
Bioactivity
Phenotype

Keywords

  • Adenovirus
  • E3 region
  • Genetic polymorphism
  • Virus egress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

No evidence of a death-like function for species B1 human adenovirus type 3 E3-9K during A549 cell line infection. / Frietze, Kathryn M.; Campos, Samuel K; Kajon, Adriana E.

In: BMC Research Notes, Vol. 5, 429, 2012.

Research output: Contribution to journalArticle

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abstract = "Background: Subspecies B1 human adenoviruses (HAdV-B1) are prevalent respiratory pathogens. Compared to their species C (HAdV-C) counterparts, relatively little work has been devoted to the characterization of their unique molecular biology. The early region 3 (E3) transcription unit is an interesting target for future efforts because of its species-specific diversity in genetic content among adenoviruses. This diversity is particularly significant for the subset of E3-encoded products that are membrane glycoproteins and may account for the distinct pathobiology of the different human adenovirus species. In order to understand the role of HAdV-B-specific genes in viral pathogenesis, we initiated the characterization of unique E3 genes. As a continuation of our efforts to define the function encoded in the highly polymorphic ORF E3-10.9K and testing the hypothesis that the E3-10.9K protein orthologs with a hydrophobic domain contribute to the efficient release of viral progeny, we generated HAdV-3 mutant viruses unable to express E3-10.9K ortholog E3-9K and examined their ability to grow, disseminate, and egress in cell culture. Results: No differences were observed in the kinetics of infected cell death, and virus progeny release or in the plaque size and dissemination phenotypes between cells infected with HAdV-3 E3-9K mutants or the parental virus. The ectopic expression of E3-10.9K orthologs with a hydrophobic domain did not compromise cell viability. Conclusions: Our data show that despite the remarkable similarities with HAdV-C E3-11.6K, HAdV-B1 ORF E3-10.9K does not encode a product with a death-like biological activity.",
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