NO prevents neutrophil-mediated pulmonary vasomotor dysfunction in acute lung injury

Randall S. Friese, David A. Fullerton, Robert C. Mcintyre, Thomas F. Rehring, Jeanette Agrafojo, Anirban Banerjee, Alden H. Harken

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The purpose of this study was to examine the effect of administration of inhaled nitric oxide (NO) on lung neutrophil accumulation and pulmonary vascular endothelial cell function in endotoxin-induced acute lung injury. Mechanically ventilated rats were studied 4 hr after endotoxin (0.5 mg/kg IP). Inhaled NO (20 ppm) was administered for either the entire 4 hr after endotoxin (continuous group) or for only the first 2 of 4 hr after endotoxin (abbreviated group). Endothelial-dependent (acetylcholine, ACh) and - independent cGMP-mediated relaxation (nitroprusside, SNP) pulmonary vasorelaxation were studied in isolated pulmonary arterial rings. Lung neutrophil accumulation was determined by myeloperoxidase assay (MPO). Inhaled NO prevented endotoxin-induced lung neutrophil accumulation as well as pulmonary endothelial cell dysfunction. However, this protection required continuous administration of inhaled NO. We conclude that inhaled NO prevents neutrophil-mediated pulmonary vascular endothelial cell dysfunction in acute lung injury.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalJournal of Surgical Research
Volume63
Issue number1
DOIs
StatePublished - Jun 1996
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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    Friese, R. S., Fullerton, D. A., Mcintyre, R. C., Rehring, T. F., Agrafojo, J., Banerjee, A., & Harken, A. H. (1996). NO prevents neutrophil-mediated pulmonary vasomotor dysfunction in acute lung injury. Journal of Surgical Research, 63(1), 23-28. https://doi.org/10.1006/jsre.1996.0216