Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: Implications for the alpine nitrogen cycle

Theodore K. Raab, David A. Lipson, Russell Monson

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Non-mycorrhizal plants of the alpine sedge, Kobresia myosuroides, take up the amino acid glycine from nutrient solutions at greater rates than NO3- or NH4+. The amino acids glutamate and proline were also taken up at high rates. Total plant biomass was twice as high after 4 months of growth on glycine, compared to NH4NO3, with significant increases in both root and leaf biomass. By taking advantage of differences in the δ13C signature of air in the growth chamber and the glycine used for growth, a two-member mixing model was used to estimate that a significant amount of the glycine was taken up as intact molecules, enough to contribute 16% of the total carbon assimilation over a 4-month growing period. Glycine uptake was inhibited when roots were exposed to N2 in place of air, and when the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) was added to the root solution. From these results it is concluded that glycine uptake occurs through active transport. Glycine uptake exhibited a Q10 of 2.0 over the temperature range 5-15° C, with relatively high rates maintained at the lowest temperature measured (5° C). Roots of Kobresia were not capable of taking up NH4+ at measureable rates. To our knowledge, this is the first report of a plant whose non-mycorrhizal roots cannot take up NH4+. Measurements of three N fractions (NO3-, NH4+, and total amino acids) in the soil pore water were made over two growing seasons in two Kobresia dry meadows using microlysimeters. At the West Knoll site, which is characterized by soils with average amounts of organic matter, the dominant forms of N in the soil pore water were NO3- and NH4+ (0-450 μmol L-1). Amino acid concentrations were generally less than 20 μmol L-1 at this site. At the East Knoll site, which is characterized by soils with higher than average amounts of organic matter, amino acids were generally present at higher concentrations (17-100 μmol L-1), compared to NO3- and NH4+. The most abundant amino acids were glycine (10-100 μmol L-1), glutamate (5-70 μmol L-1), and late in the season cysteine (5-15 μmol L-1). The results demonstrate that this sedge, which dominates dry meadow communities in many alpine ecosystems, is capable of taking up intact amino acids as a principal N source and has access to high amino acid concentrations in certain alpine soils. Such uptake of organic N may accommodate plant N demands in the face of slow alpine N mineralization rates due to cold soil temperatures.

Original languageEnglish (US)
Pages (from-to)488-494
Number of pages7
JournalOecologia
Volume108
Issue number3
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

Kobresia
nitrogen cycle
sedge
amino acid
glycine (amino acid)
uptake mechanisms
amino acids
soil pore water
glutamates
meadow
meadows
porewater
soil organic matter
soil water
cold soils
organic matter
alpine plants
soil
air
active transport

Keywords

  • Alpine plants
  • Cyperaccae
  • Nitrogen cycle
  • Organic nitrogen
  • Tundra

ASJC Scopus subject areas

  • Ecology

Cite this

Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides : Implications for the alpine nitrogen cycle. / Raab, Theodore K.; Lipson, David A.; Monson, Russell.

In: Oecologia, Vol. 108, No. 3, 11.1996, p. 488-494.

Research output: Contribution to journalArticle

@article{4de39347fe1f499495b8e86da9e7b3bc,
title = "Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: Implications for the alpine nitrogen cycle",
abstract = "Non-mycorrhizal plants of the alpine sedge, Kobresia myosuroides, take up the amino acid glycine from nutrient solutions at greater rates than NO3- or NH4+. The amino acids glutamate and proline were also taken up at high rates. Total plant biomass was twice as high after 4 months of growth on glycine, compared to NH4NO3, with significant increases in both root and leaf biomass. By taking advantage of differences in the δ13C signature of air in the growth chamber and the glycine used for growth, a two-member mixing model was used to estimate that a significant amount of the glycine was taken up as intact molecules, enough to contribute 16{\%} of the total carbon assimilation over a 4-month growing period. Glycine uptake was inhibited when roots were exposed to N2 in place of air, and when the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) was added to the root solution. From these results it is concluded that glycine uptake occurs through active transport. Glycine uptake exhibited a Q10 of 2.0 over the temperature range 5-15° C, with relatively high rates maintained at the lowest temperature measured (5° C). Roots of Kobresia were not capable of taking up NH4+ at measureable rates. To our knowledge, this is the first report of a plant whose non-mycorrhizal roots cannot take up NH4+. Measurements of three N fractions (NO3-, NH4+, and total amino acids) in the soil pore water were made over two growing seasons in two Kobresia dry meadows using microlysimeters. At the West Knoll site, which is characterized by soils with average amounts of organic matter, the dominant forms of N in the soil pore water were NO3- and NH4+ (0-450 μmol L-1). Amino acid concentrations were generally less than 20 μmol L-1 at this site. At the East Knoll site, which is characterized by soils with higher than average amounts of organic matter, amino acids were generally present at higher concentrations (17-100 μmol L-1), compared to NO3- and NH4+. The most abundant amino acids were glycine (10-100 μmol L-1), glutamate (5-70 μmol L-1), and late in the season cysteine (5-15 μmol L-1). The results demonstrate that this sedge, which dominates dry meadow communities in many alpine ecosystems, is capable of taking up intact amino acids as a principal N source and has access to high amino acid concentrations in certain alpine soils. Such uptake of organic N may accommodate plant N demands in the face of slow alpine N mineralization rates due to cold soil temperatures.",
keywords = "Alpine plants, Cyperaccae, Nitrogen cycle, Organic nitrogen, Tundra",
author = "Raab, {Theodore K.} and Lipson, {David A.} and Russell Monson",
year = "1996",
month = "11",
language = "English (US)",
volume = "108",
pages = "488--494",
journal = "Oecologia",
issn = "0029-8549",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides

T2 - Implications for the alpine nitrogen cycle

AU - Raab, Theodore K.

AU - Lipson, David A.

AU - Monson, Russell

PY - 1996/11

Y1 - 1996/11

N2 - Non-mycorrhizal plants of the alpine sedge, Kobresia myosuroides, take up the amino acid glycine from nutrient solutions at greater rates than NO3- or NH4+. The amino acids glutamate and proline were also taken up at high rates. Total plant biomass was twice as high after 4 months of growth on glycine, compared to NH4NO3, with significant increases in both root and leaf biomass. By taking advantage of differences in the δ13C signature of air in the growth chamber and the glycine used for growth, a two-member mixing model was used to estimate that a significant amount of the glycine was taken up as intact molecules, enough to contribute 16% of the total carbon assimilation over a 4-month growing period. Glycine uptake was inhibited when roots were exposed to N2 in place of air, and when the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) was added to the root solution. From these results it is concluded that glycine uptake occurs through active transport. Glycine uptake exhibited a Q10 of 2.0 over the temperature range 5-15° C, with relatively high rates maintained at the lowest temperature measured (5° C). Roots of Kobresia were not capable of taking up NH4+ at measureable rates. To our knowledge, this is the first report of a plant whose non-mycorrhizal roots cannot take up NH4+. Measurements of three N fractions (NO3-, NH4+, and total amino acids) in the soil pore water were made over two growing seasons in two Kobresia dry meadows using microlysimeters. At the West Knoll site, which is characterized by soils with average amounts of organic matter, the dominant forms of N in the soil pore water were NO3- and NH4+ (0-450 μmol L-1). Amino acid concentrations were generally less than 20 μmol L-1 at this site. At the East Knoll site, which is characterized by soils with higher than average amounts of organic matter, amino acids were generally present at higher concentrations (17-100 μmol L-1), compared to NO3- and NH4+. The most abundant amino acids were glycine (10-100 μmol L-1), glutamate (5-70 μmol L-1), and late in the season cysteine (5-15 μmol L-1). The results demonstrate that this sedge, which dominates dry meadow communities in many alpine ecosystems, is capable of taking up intact amino acids as a principal N source and has access to high amino acid concentrations in certain alpine soils. Such uptake of organic N may accommodate plant N demands in the face of slow alpine N mineralization rates due to cold soil temperatures.

AB - Non-mycorrhizal plants of the alpine sedge, Kobresia myosuroides, take up the amino acid glycine from nutrient solutions at greater rates than NO3- or NH4+. The amino acids glutamate and proline were also taken up at high rates. Total plant biomass was twice as high after 4 months of growth on glycine, compared to NH4NO3, with significant increases in both root and leaf biomass. By taking advantage of differences in the δ13C signature of air in the growth chamber and the glycine used for growth, a two-member mixing model was used to estimate that a significant amount of the glycine was taken up as intact molecules, enough to contribute 16% of the total carbon assimilation over a 4-month growing period. Glycine uptake was inhibited when roots were exposed to N2 in place of air, and when the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) was added to the root solution. From these results it is concluded that glycine uptake occurs through active transport. Glycine uptake exhibited a Q10 of 2.0 over the temperature range 5-15° C, with relatively high rates maintained at the lowest temperature measured (5° C). Roots of Kobresia were not capable of taking up NH4+ at measureable rates. To our knowledge, this is the first report of a plant whose non-mycorrhizal roots cannot take up NH4+. Measurements of three N fractions (NO3-, NH4+, and total amino acids) in the soil pore water were made over two growing seasons in two Kobresia dry meadows using microlysimeters. At the West Knoll site, which is characterized by soils with average amounts of organic matter, the dominant forms of N in the soil pore water were NO3- and NH4+ (0-450 μmol L-1). Amino acid concentrations were generally less than 20 μmol L-1 at this site. At the East Knoll site, which is characterized by soils with higher than average amounts of organic matter, amino acids were generally present at higher concentrations (17-100 μmol L-1), compared to NO3- and NH4+. The most abundant amino acids were glycine (10-100 μmol L-1), glutamate (5-70 μmol L-1), and late in the season cysteine (5-15 μmol L-1). The results demonstrate that this sedge, which dominates dry meadow communities in many alpine ecosystems, is capable of taking up intact amino acids as a principal N source and has access to high amino acid concentrations in certain alpine soils. Such uptake of organic N may accommodate plant N demands in the face of slow alpine N mineralization rates due to cold soil temperatures.

KW - Alpine plants

KW - Cyperaccae

KW - Nitrogen cycle

KW - Organic nitrogen

KW - Tundra

UR - http://www.scopus.com/inward/record.url?scp=0030301669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030301669&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030301669

VL - 108

SP - 488

EP - 494

JO - Oecologia

JF - Oecologia

SN - 0029-8549

IS - 3

ER -