Nonlinear structured illumination microscopy by surface plasmon enhanced stimulated emission depletion

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Nonlinear structured illumination microscopy (SIM) in theory has unlimited resolution over a full field of view. However under a realistic signal-to-noise ratio and a limited photon budget, the performance of nonlinear SIM strongly depends on the behavior of the nonlinear effect. Saturated SIM (SSIM) is not ideal in biological applications due to its strong photobleaching. Stimulated emission depletion (STED) SIM will have high sensitivity, higher resolution and less photo toxicity than SSIM. However, the laser power necessary to support a strong full-field STED effect is not attainable with current laser technology. We experimentally proved that surface plasmon resonance enhances (SPR) near surface STED effect by a factor of 8, and therefore STED-SIM is feasible in the total internal reflection microscopy mode with SPR enhancement. Simulation analysis predicts that SPR enhanced 2D STED is strong enough for nonlinear SIM to achieve high-speed imaging at 30-nm resolution and single molecule sensitivity. The STED-SIM superresolution microscopy method would provide a solution for observing single molecule processes in vitro or on the basal membrane of live cells.

Original languageEnglish (US)
Pages (from-to)24783-24794
Number of pages12
JournalOptics Express
Volume19
Issue number24
DOIs
StatePublished - Nov 21 2011

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stimulated emission
depletion
illumination
microscopy
surface plasmon resonance
SIM
sensitivity
toxicity
budgets
field of view
lasers
molecules
field emission
signal to noise ratios
high speed
membranes
augmentation
high resolution
photons
cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Nonlinear structured illumination microscopy by surface plasmon enhanced stimulated emission depletion. / Zhang, Han; Zhao, Ming; Peng, Leilei.

In: Optics Express, Vol. 19, No. 24, 21.11.2011, p. 24783-24794.

Research output: Contribution to journalArticle

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