Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nonlinear structured illumination microscopy (SIM) allows full-field imaging at resolutions <100 nm. Two nonlinear effects, excitation saturation (SSIM) and the photo-switching of protein had been applied to nonlinear SIM. We report a new SIM technique which utilizes the nonlinearity of STED effect. Resolution and signal noise ratio simulation shows that STED-SIM may serve as a better alternative to SSIM and SIM with photo-switchable protein. SIM requires a strong nonlinear effect in a large area. We use Surface Plasmon Resonant to enhance of evanescence field near a dielectric-metal-dielectric interface. An 8 times STED effect enhancement is achieved on an optimized glass-silver-glass- water planar structure. We further use the interference of two SPR-enhanced STED fields propagating at opposite direction to generate a 1D structured STED field. Combined with a uniform excitation field, the structure STED field allows full field total internal reflection imaging with an enhanced resolution along the structured dimension. Less than 50 nm resolution is demonstrated. A STED-SIM microscope with 2D structured STED field is under development. Future research will apply the microscope to superresolution imaging of membrane resident or near membrane structure at super-resolution in live cells.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8590
DOIs
StatePublished - 2013
EventSingle Molecule Spectroscopy and Superresolution Imaging VI - San Francisco, CA, United States
Duration: Feb 2 2013Feb 3 2013

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging VI
CountryUnited States
CitySan Francisco, CA
Period2/2/132/3/13

Fingerprint

Stimulated emission
Surface Plasmon Resonance
Surface plasmon resonance
stimulated emission
Lighting
surface plasmon resonance
Microscopy
Microscopic examination
depletion
illumination
microscopy
Imaging techniques
Glass
Microscopes
evanescence
microscopes
proteins
Proteins
membrane structures
Membrane structures

Keywords

  • SPR
  • STED
  • Structured illumination microscopy
  • superresolution

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion. / Zhang, Han; Zhao, Ming; Peng, Leilei.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8590 2013. 859011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, H, Zhao, M & Peng, L 2013, Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8590, 859011, Single Molecule Spectroscopy and Superresolution Imaging VI, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2005043
Zhang, Han ; Zhao, Ming ; Peng, Leilei. / Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8590 2013.
@inproceedings{bfa831dfc67e41e38b6f99629c6627e9,
title = "Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion",
abstract = "Nonlinear structured illumination microscopy (SIM) allows full-field imaging at resolutions <100 nm. Two nonlinear effects, excitation saturation (SSIM) and the photo-switching of protein had been applied to nonlinear SIM. We report a new SIM technique which utilizes the nonlinearity of STED effect. Resolution and signal noise ratio simulation shows that STED-SIM may serve as a better alternative to SSIM and SIM with photo-switchable protein. SIM requires a strong nonlinear effect in a large area. We use Surface Plasmon Resonant to enhance of evanescence field near a dielectric-metal-dielectric interface. An 8 times STED effect enhancement is achieved on an optimized glass-silver-glass- water planar structure. We further use the interference of two SPR-enhanced STED fields propagating at opposite direction to generate a 1D structured STED field. Combined with a uniform excitation field, the structure STED field allows full field total internal reflection imaging with an enhanced resolution along the structured dimension. Less than 50 nm resolution is demonstrated. A STED-SIM microscope with 2D structured STED field is under development. Future research will apply the microscope to superresolution imaging of membrane resident or near membrane structure at super-resolution in live cells.",
keywords = "SPR, STED, Structured illumination microscopy, superresolution",
author = "Han Zhang and Ming Zhao and Leilei Peng",
year = "2013",
doi = "10.1117/12.2005043",
language = "English (US)",
isbn = "9780819493590",
volume = "8590",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion

AU - Zhang, Han

AU - Zhao, Ming

AU - Peng, Leilei

PY - 2013

Y1 - 2013

N2 - Nonlinear structured illumination microscopy (SIM) allows full-field imaging at resolutions <100 nm. Two nonlinear effects, excitation saturation (SSIM) and the photo-switching of protein had been applied to nonlinear SIM. We report a new SIM technique which utilizes the nonlinearity of STED effect. Resolution and signal noise ratio simulation shows that STED-SIM may serve as a better alternative to SSIM and SIM with photo-switchable protein. SIM requires a strong nonlinear effect in a large area. We use Surface Plasmon Resonant to enhance of evanescence field near a dielectric-metal-dielectric interface. An 8 times STED effect enhancement is achieved on an optimized glass-silver-glass- water planar structure. We further use the interference of two SPR-enhanced STED fields propagating at opposite direction to generate a 1D structured STED field. Combined with a uniform excitation field, the structure STED field allows full field total internal reflection imaging with an enhanced resolution along the structured dimension. Less than 50 nm resolution is demonstrated. A STED-SIM microscope with 2D structured STED field is under development. Future research will apply the microscope to superresolution imaging of membrane resident or near membrane structure at super-resolution in live cells.

AB - Nonlinear structured illumination microscopy (SIM) allows full-field imaging at resolutions <100 nm. Two nonlinear effects, excitation saturation (SSIM) and the photo-switching of protein had been applied to nonlinear SIM. We report a new SIM technique which utilizes the nonlinearity of STED effect. Resolution and signal noise ratio simulation shows that STED-SIM may serve as a better alternative to SSIM and SIM with photo-switchable protein. SIM requires a strong nonlinear effect in a large area. We use Surface Plasmon Resonant to enhance of evanescence field near a dielectric-metal-dielectric interface. An 8 times STED effect enhancement is achieved on an optimized glass-silver-glass- water planar structure. We further use the interference of two SPR-enhanced STED fields propagating at opposite direction to generate a 1D structured STED field. Combined with a uniform excitation field, the structure STED field allows full field total internal reflection imaging with an enhanced resolution along the structured dimension. Less than 50 nm resolution is demonstrated. A STED-SIM microscope with 2D structured STED field is under development. Future research will apply the microscope to superresolution imaging of membrane resident or near membrane structure at super-resolution in live cells.

KW - SPR

KW - STED

KW - Structured illumination microscopy

KW - superresolution

UR - http://www.scopus.com/inward/record.url?scp=84878070077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878070077&partnerID=8YFLogxK

U2 - 10.1117/12.2005043

DO - 10.1117/12.2005043

M3 - Conference contribution

AN - SCOPUS:84878070077

SN - 9780819493590

VL - 8590

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -