Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor

Cortnie L. Hartwig, Jason Worrell, Richard B Levine, Mani Ramaswami, Subhabrata Sanyal

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

During learning and memory formation, information flow through networks is regulated significantly through structural alterations in neurons. Dendrites, sites of signal integration, are key targets of activity-mediated modifications. Although local mechanisms of dendritic growth ensure synapse-specific changes, global mechanisms linking neural activity to nuclear gene expression may have profound influences on neural function. Fos, being an immediate-early gene, is ideally suited to be an initial transducer of neural activity, but a precise role for the AP-1 transcription factor in dendrite growth remains to be elucidated. Here we measure changes in the dendritic fields of identified Drosophila motor neurons in vivo and in primary culture to investigate the role of the immediate-early transcription factor AP-1 in regulating endogenous and activity-induced dendrite growth. Our data indicate that (a) increased neural excitability or depolarization stimulates dendrite growth, (b) AP-1 (a Fos, Jun heterodimer) is required for normal motor neuron dendritic growth during development and in response to activity induction, and (c) neuronal Fos protein levels are rapidly but transiently induced in motor neurons following neural activity. Taken together, these results show that AP-1 mediated transcription is important for dendrite growth, and that neural activity influences global dendritic growth through a gene-expression dependent mechanism gated by AP-I.

Original languageEnglish (US)
Pages (from-to)1225-1242
Number of pages18
JournalDevelopmental Neurobiology
Volume68
Issue number10
DOIs
StatePublished - Sep 1 2008

Fingerprint

Transcription Factor AP-1
Motor Neurons
Dendrites
Drosophila
Growth
Proto-Oncogene Proteins c-fos
Gene Expression
Immediate-Early Genes
Transducers
Growth and Development
Synapses
Learning
Neurons

Keywords

  • AP-1
  • Dendrites
  • Development
  • Drosophila
  • Plasticity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor. / Hartwig, Cortnie L.; Worrell, Jason; Levine, Richard B; Ramaswami, Mani; Sanyal, Subhabrata.

In: Developmental Neurobiology, Vol. 68, No. 10, 01.09.2008, p. 1225-1242.

Research output: Contribution to journalArticle

Hartwig, Cortnie L. ; Worrell, Jason ; Levine, Richard B ; Ramaswami, Mani ; Sanyal, Subhabrata. / Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor. In: Developmental Neurobiology. 2008 ; Vol. 68, No. 10. pp. 1225-1242.
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