Norovirus narita 104 virus-like particles expressed in nicotiana benthamiana induce serum and mucosal immune responses

Lolita George Mathew, Melissa Herbst-Kralovetz, Hugh S. Mason

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Narita 104 virus is a human pathogen belonging to the norovirus (family Caliciviridae) genogroup II. Noroviruses cause epidemic gastroenteritis worldwide. To explore the potential of developing a plant-based vaccine, a plant optimized gene encoding Narita 104 virus capsid protein (NaVCP) was expressed transiently in Nicotiana benthamiana using a tobacco mosaic virus expression system. NaVCP accumulated up to approximately 0.3 mg/g fresh weight of leaf at 4 days postinfection. Initiation of hypersensitive response-like symptoms followed by tissue necrosis necessitated a brief infection time and was a significant factor limiting expression. Transmission electron microscopy of plant-derived NaVCP confirmed the presence of fully assembled virus-like particles (VLPs). In this study, an optimized method to express and partially purify NaVCP is described. Further, partially purified NaVCP was used to immunize mice by intranasal delivery and generated significant mucosal and serum antibody responses. Thus, plant-derived Narita 104 VLPs have potential for use as a candidate subunit vaccine or as a component of a multivalent subunit vaccine, along with other genotype-specific plant-derived VLPs.

Original languageEnglish (US)
Article number807539
JournalBioMed Research International
Volume2014
DOIs
StatePublished - 2014

Fingerprint

Norovirus
Mucosal Immunity
Viruses
Virion
Capsid Proteins
Tobacco
Serum
Subunit Vaccines
Caliciviridae
Genotype
Tobacco Mosaic Virus
Plant Viruses
Plant Genes
Gastroenteritis
Transmission Electron Microscopy
Antibody Formation
Necrosis
Vaccines
Gene encoding
Weights and Measures

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Norovirus narita 104 virus-like particles expressed in nicotiana benthamiana induce serum and mucosal immune responses. / Mathew, Lolita George; Herbst-Kralovetz, Melissa; Mason, Hugh S.

In: BioMed Research International, Vol. 2014, 807539, 2014.

Research output: Contribution to journalArticle

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