Novel, closely related, white spot syndrome virus (WSSV) genotypes from Madagascar, Mozambique and the Kingdom of Saudi Arabia

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Abstract

White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the aquaculture industry around the world. During 2010 to 2012, WSSV caused severe mortalities in cultured penaeid shrimp in Saudi Arabia, Mozambique and Madagascar. To investigate the origins of these WSSV, we performed genotyping analyses at 5 loci: the 3 open reading frames (ORFs) 125, 94 and 75, each containing a variable number of tandem repeats (VNTR), and deletions in the 2 variable regions, VR14/15 and VR23/24. We categorized the WSSV genotype as {N125, N 94, N75, δX14/15, δX 23/24} where N is the number of repeat units in a specific ORF and δX is the length (base pair) of deletion within the variable region. We detected 4 WSSV genotypes, which were characterized by a full-length deletion in ORF94/95, a relatively small ORF75 and one specific deletion length in each variable region. There are 2 closely related genotypes in these 3 countries: {6125, del94, 375, δ595014/15, δ1097123/24} and {7125, del94, 375, δ595014/15, δ1097123/24}, where del is the full-length ORF deletion. In Saudi Arabia, 2 other related types of WSSV were also found: {6125, 794, 375, δ595014/15, δ1097 123/24} and {8125, 1394, 375, δ595014/15, δ1097123/24}. The identical patterns of 3 loci in these 4 types indicate that they have a common lineage, and this suggests that the WSSV epidemics in these 3 countries were from a common source, possibly the environment.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalDiseases of Aquatic Organisms
Volume106
Issue number1
DOIs
StatePublished - Sep 24 2013

Fingerprint

White spot syndrome virus
Mozambique
Saudi Arabia
Madagascar
virus
genotype
open reading frames
Penaeidae
aquaculture industry
loci
minisatellite repeats
genotyping
mortality
economics

Keywords

  • Africa
  • Saudi Arabia
  • Variable number of tandem repeats
  • Variablelength deletion
  • VNTR analysis
  • WSSV genotyping

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

@article{7dcf1849edbd41459eff8a5463c72405,
title = "Novel, closely related, white spot syndrome virus (WSSV) genotypes from Madagascar, Mozambique and the Kingdom of Saudi Arabia",
abstract = "White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the aquaculture industry around the world. During 2010 to 2012, WSSV caused severe mortalities in cultured penaeid shrimp in Saudi Arabia, Mozambique and Madagascar. To investigate the origins of these WSSV, we performed genotyping analyses at 5 loci: the 3 open reading frames (ORFs) 125, 94 and 75, each containing a variable number of tandem repeats (VNTR), and deletions in the 2 variable regions, VR14/15 and VR23/24. We categorized the WSSV genotype as {N125, N 94, N75, δX14/15, δX 23/24} where N is the number of repeat units in a specific ORF and δX is the length (base pair) of deletion within the variable region. We detected 4 WSSV genotypes, which were characterized by a full-length deletion in ORF94/95, a relatively small ORF75 and one specific deletion length in each variable region. There are 2 closely related genotypes in these 3 countries: {6125, del94, 375, δ595014/15, δ1097123/24} and {7125, del94, 375, δ595014/15, δ1097123/24}, where del is the full-length ORF deletion. In Saudi Arabia, 2 other related types of WSSV were also found: {6125, 794, 375, δ595014/15, δ1097 123/24} and {8125, 1394, 375, δ595014/15, δ1097123/24}. The identical patterns of 3 loci in these 4 types indicate that they have a common lineage, and this suggests that the WSSV epidemics in these 3 countries were from a common source, possibly the environment.",
keywords = "Africa, Saudi Arabia, Variable number of tandem repeats, Variablelength deletion, VNTR analysis, WSSV genotyping",
author = "Feng-Jyu Tang-Nelson and {Le Groumellec}, Marc and Lightner, {Donald V}",
year = "2013",
month = "9",
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doi = "10.3354/dao02645",
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volume = "106",
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T1 - Novel, closely related, white spot syndrome virus (WSSV) genotypes from Madagascar, Mozambique and the Kingdom of Saudi Arabia

AU - Tang-Nelson, Feng-Jyu

AU - Le Groumellec, Marc

AU - Lightner, Donald V

PY - 2013/9/24

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N2 - White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the aquaculture industry around the world. During 2010 to 2012, WSSV caused severe mortalities in cultured penaeid shrimp in Saudi Arabia, Mozambique and Madagascar. To investigate the origins of these WSSV, we performed genotyping analyses at 5 loci: the 3 open reading frames (ORFs) 125, 94 and 75, each containing a variable number of tandem repeats (VNTR), and deletions in the 2 variable regions, VR14/15 and VR23/24. We categorized the WSSV genotype as {N125, N 94, N75, δX14/15, δX 23/24} where N is the number of repeat units in a specific ORF and δX is the length (base pair) of deletion within the variable region. We detected 4 WSSV genotypes, which were characterized by a full-length deletion in ORF94/95, a relatively small ORF75 and one specific deletion length in each variable region. There are 2 closely related genotypes in these 3 countries: {6125, del94, 375, δ595014/15, δ1097123/24} and {7125, del94, 375, δ595014/15, δ1097123/24}, where del is the full-length ORF deletion. In Saudi Arabia, 2 other related types of WSSV were also found: {6125, 794, 375, δ595014/15, δ1097 123/24} and {8125, 1394, 375, δ595014/15, δ1097123/24}. The identical patterns of 3 loci in these 4 types indicate that they have a common lineage, and this suggests that the WSSV epidemics in these 3 countries were from a common source, possibly the environment.

AB - White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the aquaculture industry around the world. During 2010 to 2012, WSSV caused severe mortalities in cultured penaeid shrimp in Saudi Arabia, Mozambique and Madagascar. To investigate the origins of these WSSV, we performed genotyping analyses at 5 loci: the 3 open reading frames (ORFs) 125, 94 and 75, each containing a variable number of tandem repeats (VNTR), and deletions in the 2 variable regions, VR14/15 and VR23/24. We categorized the WSSV genotype as {N125, N 94, N75, δX14/15, δX 23/24} where N is the number of repeat units in a specific ORF and δX is the length (base pair) of deletion within the variable region. We detected 4 WSSV genotypes, which were characterized by a full-length deletion in ORF94/95, a relatively small ORF75 and one specific deletion length in each variable region. There are 2 closely related genotypes in these 3 countries: {6125, del94, 375, δ595014/15, δ1097123/24} and {7125, del94, 375, δ595014/15, δ1097123/24}, where del is the full-length ORF deletion. In Saudi Arabia, 2 other related types of WSSV were also found: {6125, 794, 375, δ595014/15, δ1097 123/24} and {8125, 1394, 375, δ595014/15, δ1097123/24}. The identical patterns of 3 loci in these 4 types indicate that they have a common lineage, and this suggests that the WSSV epidemics in these 3 countries were from a common source, possibly the environment.

KW - Africa

KW - Saudi Arabia

KW - Variable number of tandem repeats

KW - Variablelength deletion

KW - VNTR analysis

KW - WSSV genotyping

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