Novel discovery of LYVE-1 expression in the hyaloid vascular system

Hui Zhang, Julie Tse, Xuemei Hu, Marlys H Witte, Michael J Bernas, Jinjoo Kang, Firehiwott Tilahun, Young Kwon Hong, Mengsheng Qiu, Lu Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

PURPOSE. The hyaloid vascular system (HVS) is a transient network nourishing developing eyes and has been widely used as a natural model to study blood vessel regression. Failure of its regression in humans leads to several blinding diseases. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a recently defined lymphatic marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the HVS. This study was conducted to investigate whether LYVE-1 is expressed in the HVS and how it is associated with the vascular structure and macrophage phenotype. METHODS. Normal C57BL/6 mouse eyeballs were sampled from embryonic day (E) 10.5 to postnatal (P) and adult stages for immunofluorescent microscopic studies with antibodies against LYVE-1, CD31 (panendothelial cell marker), and F4/80 (macrophage marker). Additionally, Angiopoietin-2 (Ang-2) knockout mice with abnormally persistent HVS were examined. RESULTS. The LYVE-1 expression was detected on normal HVS between E12.5 and P14. The LYVE-1 + cells were F4/80 + but CD31 -, indicating a macrophage lineage. Additionally, LYVE-1 + cells bud on CD31 +vessels and constitute an integral part of the network in both normal developing and Ang-2 knockout mice. CONCLUSIONS. This study provides the first evidence that the HVS contains a LYVE-1 + cellular component in both physiological and pathologic conditions. This novel finding not only provides a new concept in defining the embryogenesis and pathogenesis of the HVS, it also leads to a completely natural model in which to study the functions of the LYVE-1 pathway, an important topic for lymphatic research as well.

Original languageEnglish (US)
Pages (from-to)6157-6161
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume51
Issue number12
DOIs
StatePublished - Dec 2010

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Blood Vessels
Macrophages
Angiopoietin-2
Knockout Mice
Lymphatic Vessels
Hyaluronic Acid
Inbred C57BL Mouse
Embryonic Development
Phenotype
Antibodies
Research

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Novel discovery of LYVE-1 expression in the hyaloid vascular system. / Zhang, Hui; Tse, Julie; Hu, Xuemei; Witte, Marlys H; Bernas, Michael J; Kang, Jinjoo; Tilahun, Firehiwott; Hong, Young Kwon; Qiu, Mengsheng; Chen, Lu.

In: Investigative Ophthalmology and Visual Science, Vol. 51, No. 12, 12.2010, p. 6157-6161.

Research output: Contribution to journalArticle

Zhang, H, Tse, J, Hu, X, Witte, MH, Bernas, MJ, Kang, J, Tilahun, F, Hong, YK, Qiu, M & Chen, L 2010, 'Novel discovery of LYVE-1 expression in the hyaloid vascular system', Investigative Ophthalmology and Visual Science, vol. 51, no. 12, pp. 6157-6161. https://doi.org/10.1167/iovs.10-5205
Zhang, Hui ; Tse, Julie ; Hu, Xuemei ; Witte, Marlys H ; Bernas, Michael J ; Kang, Jinjoo ; Tilahun, Firehiwott ; Hong, Young Kwon ; Qiu, Mengsheng ; Chen, Lu. / Novel discovery of LYVE-1 expression in the hyaloid vascular system. In: Investigative Ophthalmology and Visual Science. 2010 ; Vol. 51, No. 12. pp. 6157-6161.
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abstract = "PURPOSE. The hyaloid vascular system (HVS) is a transient network nourishing developing eyes and has been widely used as a natural model to study blood vessel regression. Failure of its regression in humans leads to several blinding diseases. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a recently defined lymphatic marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the HVS. This study was conducted to investigate whether LYVE-1 is expressed in the HVS and how it is associated with the vascular structure and macrophage phenotype. METHODS. Normal C57BL/6 mouse eyeballs were sampled from embryonic day (E) 10.5 to postnatal (P) and adult stages for immunofluorescent microscopic studies with antibodies against LYVE-1, CD31 (panendothelial cell marker), and F4/80 (macrophage marker). Additionally, Angiopoietin-2 (Ang-2) knockout mice with abnormally persistent HVS were examined. RESULTS. The LYVE-1 expression was detected on normal HVS between E12.5 and P14. The LYVE-1 + cells were F4/80 + but CD31 -, indicating a macrophage lineage. Additionally, LYVE-1 + cells bud on CD31 +vessels and constitute an integral part of the network in both normal developing and Ang-2 knockout mice. CONCLUSIONS. This study provides the first evidence that the HVS contains a LYVE-1 + cellular component in both physiological and pathologic conditions. This novel finding not only provides a new concept in defining the embryogenesis and pathogenesis of the HVS, it also leads to a completely natural model in which to study the functions of the LYVE-1 pathway, an important topic for lymphatic research as well.",
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AU - Tse, Julie

AU - Hu, Xuemei

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AU - Bernas, Michael J

AU - Kang, Jinjoo

AU - Tilahun, Firehiwott

AU - Hong, Young Kwon

AU - Qiu, Mengsheng

AU - Chen, Lu

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AB - PURPOSE. The hyaloid vascular system (HVS) is a transient network nourishing developing eyes and has been widely used as a natural model to study blood vessel regression. Failure of its regression in humans leads to several blinding diseases. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a recently defined lymphatic marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the HVS. This study was conducted to investigate whether LYVE-1 is expressed in the HVS and how it is associated with the vascular structure and macrophage phenotype. METHODS. Normal C57BL/6 mouse eyeballs were sampled from embryonic day (E) 10.5 to postnatal (P) and adult stages for immunofluorescent microscopic studies with antibodies against LYVE-1, CD31 (panendothelial cell marker), and F4/80 (macrophage marker). Additionally, Angiopoietin-2 (Ang-2) knockout mice with abnormally persistent HVS were examined. RESULTS. The LYVE-1 expression was detected on normal HVS between E12.5 and P14. The LYVE-1 + cells were F4/80 + but CD31 -, indicating a macrophage lineage. Additionally, LYVE-1 + cells bud on CD31 +vessels and constitute an integral part of the network in both normal developing and Ang-2 knockout mice. CONCLUSIONS. This study provides the first evidence that the HVS contains a LYVE-1 + cellular component in both physiological and pathologic conditions. This novel finding not only provides a new concept in defining the embryogenesis and pathogenesis of the HVS, it also leads to a completely natural model in which to study the functions of the LYVE-1 pathway, an important topic for lymphatic research as well.

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