Novel eceriferum mutants in Arabidopsis thaliana

Aaron M. Rashotte, Matthew A. Jenks, Amanda S. Ross, Kenneth A Feldmann

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We conducted a novel non-visual screen for cuticular wax mutants in Arabidopsis thaliana (L.) Heynh. Using gas chromatography we screened over 1,200 ethyl methane sulfonate (EMS)-mutagenized lines for alterations in the major A. thaliana wild-type stem cuticular chemicals. Five lines showed distinct differences from the wild type and were further analyzed by gas chromatography and scanning electron microscopy. The five mutants were mapped to specific chromosome locations and tested for allelism with other wax mutant loci mapping to the same region. Toward this end, the mapping of the cuticular wax (cer) mutants cer10 to cer20 was conducted to allow more efficient allelism tests with newly identified lines. From these five lines, we have identified three mutants defining novel genes that have been designated CER22, CER23, and CER24. Detailed stem and leaf chemistry has allowed us to place these novel mutants in specific steps of the cuticular wax biosynthetic pathway and to make hypotheses about the function of their gene products.

Original languageEnglish (US)
Pages (from-to)5-13
Number of pages9
JournalPlanta
Volume219
Issue number1
DOIs
StatePublished - May 2004

Fingerprint

Waxes
Arabidopsis
Arabidopsis thaliana
mutants
epicuticular wax
Gas Chromatography
allelism
Genetic Complementation Test
Biosynthetic Pathways
Methane
gas chromatography
Electron Scanning Microscopy
Genes
ethyl methanesulfonate
stems
Chromosomes
waxes
biochemical pathways
chemistry
scanning electron microscopy

Keywords

  • Arabidopsis
  • cer
  • Cuticular wax
  • eceriferum

ASJC Scopus subject areas

  • Plant Science

Cite this

Novel eceriferum mutants in Arabidopsis thaliana. / Rashotte, Aaron M.; Jenks, Matthew A.; Ross, Amanda S.; Feldmann, Kenneth A.

In: Planta, Vol. 219, No. 1, 05.2004, p. 5-13.

Research output: Contribution to journalArticle

Rashotte, Aaron M. ; Jenks, Matthew A. ; Ross, Amanda S. ; Feldmann, Kenneth A. / Novel eceriferum mutants in Arabidopsis thaliana. In: Planta. 2004 ; Vol. 219, No. 1. pp. 5-13.
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