Nrf2/Maf-binding-site-containing functional Cyp6a2 allele is associated with DDT resistance in Drosophila melanogaster

Hua Wan, Yan Liu, Mei Li, Shunyi Zhu, Xianchun Li, Barry R. Pittendrigh, Xinghui Qiu

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

BACKGROUND: Increased insecticide detoxification mediated by cytochrome P450s is a common mechanism of insecticide resistance. Although Cyp6a2 has been observed to be overexpressed in many 4,4'-dichlorodiphenyltrichloroethane (DDT)-resistant strains of Drosophilamelanogaster, how Cyp6a2 is regulated and whether its overproduction confers DDT resistance remain elusive. RESULTS: Molecular analysis identified five Cyp6a2 alleles (Cyp6a2Canton-S-1, Cyp6a2Canton-S-2, Cyp6a291-C, Cyp6a291-R and Cyp6a2Wisconsin-WD) from four D.melanogaster strains, notably differing in the presence or absence of an intact Nrf2/Maf (a transcription factor) binding site in the 5'-promoter core region, a 'G1410' frameshift deletion mutation in the heme-binding region and a long terminal repeat (LTR) of transposable element 17.6 in the 3'-untranslated region (UTR). Linkage analysis confirmed that DDT resistance was genetically linked to a Nrf2/Maf-binding-site-containing, LTR-lacking functional allele of Cyp6a2 (Cyp6a291-R). The qRT-PCR results showed that overexpression of functional Cyp6a2 was consistently associated with DDT resistance. Luciferase reporter gene assays revealed that an intact Nrf2/Maf binding site in the 5'-promoter core region enhanced the constitutive transcription of Cyp6a2. CONCLUSION: The results suggest that the Nrf2/Maf binding-site-containing functional Cyp6a2allele is associated with DDT resistance in the D.melanogaster strains under study.

Original languageEnglish (US)
Pages (from-to)1048-1058
Number of pages11
JournalPest management science
Volume70
Issue number7
DOIs
StatePublished - Jul 2014

Keywords

  • Cyp6a2
  • DDT resistance
  • Frameshift mutation
  • Long terminal repeat
  • Nrf2/Maf binding site
  • Overexpression

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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