N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules

William H Dantzler, O. H. Brokl

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

N1-methylnicotinamide (NMN) transport was studied in isolated perfused snake (Thamnophis spp.) proximal renal tubules. Unidirectional lumen-to-bath (J(NMN)(l→b)) and bath-to-lumen (J(NMN)(b→l)) fluxes saturated. Although J(NMN)(l→b) and J(NMN)(b→l) were similar, mean J(NMN)(l→b) tended to exceed mean J(NMN)(b→l) at all concentrations studied. Direct measurements confirmed a net reabsorptive flux equal to the difference between the unidirectional fluxes. This transport, opposite in direction to tetraethylammonium (TEA) transport, was not inhibited by TEA. Transport into the cells across both the luminal and peritubular membranes during flux measurements was apparently down an electrochemical gradient by a mediated process that was sodium dependent. Inhibition with NMN analogues suggested that transport into the cells across the luminal membrane during J(NMN)(l→b) was more specific than transport into the cells across the peritubular membrane during J(NMN)(b→l). Transport out of the cells across both the luminal and peritubular membranes during flux measurements was apparently against an electrochemical gradient.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume250
Edition3
StatePublished - 1986

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Proximal Kidney Tubule
Snakes
Tetraethylammonium
Membranes
Baths
Colubridae
Sodium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dantzler, W. H., & Brokl, O. H. (1986). N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (3 ed., Vol. 250)

N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules. / Dantzler, William H; Brokl, O. H.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 250 3. ed. 1986.

Research output: Chapter in Book/Report/Conference proceedingChapter

Dantzler, WH & Brokl, OH 1986, N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 3 edn, vol. 250.
Dantzler WH, Brokl OH. N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 3 ed. Vol. 250. 1986
Dantzler, William H ; Brokl, O. H. / N1-methylnicotinamide transport by isolated perfused snake proximal renal tubules. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 250 3. ed. 1986.
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