N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis

Stephen J. Anderson, Marianne C. Kramer, Sager J. Gosai, Xiang Yu, Lee E. Vandivier, Andrew D.L. Nelson, Zachary D. Anderson, Mark A Beilstein, Rupert G. Fray, Eric H Lyons, Brian D. Gregory

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

N6-methyladenosine (m6A) is a dynamic, reversible, covalently modified ribonucleotide that occurs predominantly toward 3′ ends of eukaryotic mRNAs and is essential for their proper function and regulation. In Arabidopsis thaliana, many RNAs contain at least one m6A site, yet the transcriptome-wide function of m6A remains mostly unknown. Here, we show that many m6A-modified mRNAs in Arabidopsis have reduced abundance in the absence of this mark. The decrease in abundance is due to transcript destabilization caused by cleavage occurring 4 or 5 nt directly upstream of unmodified m6A sites. Importantly, we also find that, upon agriculturally relevant salt treatment, m6A is dynamically deposited on and stabilizes transcripts encoding proteins required for salt and osmotic stress response. Overall, our findings reveal that m6A generally acts as a stabilizing mark through inhibition of site-specific cleavage in plant transcriptomes, and this mechanism is required for proper regulation of the salt-stress-responsive transcriptome. N6-methyladenosine (m6A) is the most prevalent internal covalent mRNA modification and is essential for proper function and regulation of the transcriptome. Using multiple transcriptome-wide approaches, Anderson et al. reveal that, in plant somatic tissue, m6A stabilizes transcripts by inhibiting ribonucleolytic cleavage directly upstream of these modification sites.

Original languageEnglish (US)
Pages (from-to)1146-1157.e3
JournalCell Reports
Volume25
Issue number5
DOIs
StatePublished - Oct 30 2018

Fingerprint

Transcriptome
Arabidopsis
Salts
Messenger RNA
Ribonucleotides
Osmoregulation
RNA
Tissue
Proteins

Keywords

  • mA
  • mRNA
  • N-methyladenosine
  • ribonuclease
  • RNA cleavage
  • RNA covalent modifications
  • RNA degradation
  • RNA stability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Anderson, S. J., Kramer, M. C., Gosai, S. J., Yu, X., Vandivier, L. E., Nelson, A. D. L., ... Gregory, B. D. (2018). N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. Cell Reports, 25(5), 1146-1157.e3. https://doi.org/10.1016/j.celrep.2018.10.020

N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. / Anderson, Stephen J.; Kramer, Marianne C.; Gosai, Sager J.; Yu, Xiang; Vandivier, Lee E.; Nelson, Andrew D.L.; Anderson, Zachary D.; Beilstein, Mark A; Fray, Rupert G.; Lyons, Eric H; Gregory, Brian D.

In: Cell Reports, Vol. 25, No. 5, 30.10.2018, p. 1146-1157.e3.

Research output: Contribution to journalArticle

Anderson, SJ, Kramer, MC, Gosai, SJ, Yu, X, Vandivier, LE, Nelson, ADL, Anderson, ZD, Beilstein, MA, Fray, RG, Lyons, EH & Gregory, BD 2018, 'N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis', Cell Reports, vol. 25, no. 5, pp. 1146-1157.e3. https://doi.org/10.1016/j.celrep.2018.10.020
Anderson SJ, Kramer MC, Gosai SJ, Yu X, Vandivier LE, Nelson ADL et al. N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. Cell Reports. 2018 Oct 30;25(5):1146-1157.e3. https://doi.org/10.1016/j.celrep.2018.10.020
Anderson, Stephen J. ; Kramer, Marianne C. ; Gosai, Sager J. ; Yu, Xiang ; Vandivier, Lee E. ; Nelson, Andrew D.L. ; Anderson, Zachary D. ; Beilstein, Mark A ; Fray, Rupert G. ; Lyons, Eric H ; Gregory, Brian D. / N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. In: Cell Reports. 2018 ; Vol. 25, No. 5. pp. 1146-1157.e3.
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