Nuclear and cytoplasmic receptors for 1,25-dihydroxycholecalciferol in intestinal mucosa

Peter F. Brumbaugh, Mark R. Haussler

Research output: Contribution to journalArticle

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Abstract

The apparent hormonal form of cholecalciferol, 1,25-dihydroxycholecalciferol (1,25-(OH)2-CC), was incubated with intestinal mucosa homogenates and whole intestinal tissue, in vitro. After 40-70 min, 1,25-(OH)2-CC was specifically associated with the nuclear chromatin fraction. This sterol remains bound to the cytosol fraction at 0°C and a dramatic movement to the nuclear chromatin occurs at 37°C indicating that the subcellular localization of the sterol is temperature dependent. Isolated intestinal cytosol, previously incubated with 1,25-(OH)2-CC, is required for transportation of the hormone to the intestinal chromatin fraction; cytosol fractions from other tissues are ineffective mediators of this sterol migration. It is concluded that the intestinal cytosol contains a specific receptor that functions to transport 1,25-(OH)2-CC to the nucleus, its probable site of action.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume51
Issue number1
DOIs
StatePublished - Mar 5 1973

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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