Nuclear chromatin patterns in normal, hyperplastic and atypical endometrium

Francisco A Garcia, J. R. Davis, David S Alberts, Kenneth D Hatch, B. Weyn, D. Thompson, P. H. Bartels

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: To characterize the nuclei of endometrial lesions for the diagnostic categories of normal glandular tissue, simple hyperplasia, atypical hyperplasia and adenocarcinoma of the endometrium, with the specific goal of probing for heterogeneity. STUDY DESIGN: For each diagnostic category the images of 360 nuclei were recorded on a high-resolution video microphotometer. Features descriptive of the statistical and spatial distribution of nuclear chromatin were computed for each nucleus. A nonsupervised learning algorithm, P-index, was employed to establish subsets of nuclei within each diagnostic category and to determine whether these subsets were statistically significantly different in the nuclear chromatin pattern. RESULTS: Lesions from cases of hyperplasia, atypical hyperplasia and adenocarcinoma of the endometrium each contained several subsets of nuclei with statistically significantly different chromatin patterns. For one such subset from each diagnostic category, a clear trend of progression toward adenocarcinoma could be demonstrated. CONCLUSION: The nuclei in endometrial lesions represent a highly heterogeneous set. Any measure of lesion progression or regression due to chemopreventive intervention, in an individual case, will have to examine the proportion of nuclei in each of these subsets as well as measures of deviation from normal for each subset.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume23
Issue number2
StatePublished - 2001

Fingerprint

Endometrium
Chromatin
Hyperplasia
Adenocarcinoma
Statistical Distributions
Learning

Keywords

  • Cell nucleus
  • Chromatin
  • Endometrial neoplasms

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology

Cite this

Nuclear chromatin patterns in normal, hyperplastic and atypical endometrium. / Garcia, Francisco A; Davis, J. R.; Alberts, David S; Hatch, Kenneth D; Weyn, B.; Thompson, D.; Bartels, P. H.

In: Analytical and Quantitative Cytology and Histology, Vol. 23, No. 2, 2001, p. 144-150.

Research output: Contribution to journalArticle

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AU - Davis, J. R.

AU - Alberts, David S

AU - Hatch, Kenneth D

AU - Weyn, B.

AU - Thompson, D.

AU - Bartels, P. H.

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AB - OBJECTIVE: To characterize the nuclei of endometrial lesions for the diagnostic categories of normal glandular tissue, simple hyperplasia, atypical hyperplasia and adenocarcinoma of the endometrium, with the specific goal of probing for heterogeneity. STUDY DESIGN: For each diagnostic category the images of 360 nuclei were recorded on a high-resolution video microphotometer. Features descriptive of the statistical and spatial distribution of nuclear chromatin were computed for each nucleus. A nonsupervised learning algorithm, P-index, was employed to establish subsets of nuclei within each diagnostic category and to determine whether these subsets were statistically significantly different in the nuclear chromatin pattern. RESULTS: Lesions from cases of hyperplasia, atypical hyperplasia and adenocarcinoma of the endometrium each contained several subsets of nuclei with statistically significantly different chromatin patterns. For one such subset from each diagnostic category, a clear trend of progression toward adenocarcinoma could be demonstrated. CONCLUSION: The nuclei in endometrial lesions represent a highly heterogeneous set. Any measure of lesion progression or regression due to chemopreventive intervention, in an individual case, will have to examine the proportion of nuclei in each of these subsets as well as measures of deviation from normal for each subset.

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