Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry

J. K. Brown, G. M. Lambert, M. Ghanim, H. Czosnek, D. W. Galbraith

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius) was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae) as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp) indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.

Original languageEnglish (US)
Pages (from-to)309-312
Number of pages4
JournalBulletin of Entomological Research
Volume95
Issue number4
DOIs
StatePublished - Aug 1 2005

Keywords

  • Bemisia tabaci
  • Flow cytometry
  • Genome
  • Hemiptera
  • Nuclear DNA

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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