Nuclear dynamics in Arabidopsis thaliana

E. Chytilova, J. Macas, E. Sliwinska, S. M. Rafelski, G. M. Lambert, David W Galbraith

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The nucleus is a definitive feature of eukaryotic cells, comprising twin bilamellar membranes, the inner and outer nuclear membranes, which separate the nucleoplasmic and cytoplasmic compartments. Nuclear pores, complex macromolecular assemblies that connect the two membranes, mediate communication between these compartments. To explore the morphology, topology, and dynamics of nuclei within living plant cells, we have developed a novel method of confocal laser scanning fluorescence microscopy under time-lapse conditions. This is used for the examination of the transgenic expression in Arabidopsis thaliana of a chimeric protein, comprising the GFP (Green-Fluorescent Protein of Aequorea victoria) translationally fused to an effective nuclear localization signal (NLS) and to β-glucuronidase (GUS) from E. coli. This large protein is targeted to the nucleus and accumulates exclusively within the nucleoplasm. This article provides online access to movies that illustrate the remarkable and unusual properties displayed by the nuclei, including polymorphic shape changes and rapid, long-distance, intracellular movement. Movement is mediated by actin but not by tubulin; it therefore appears distinct from mechanisms of nuclear positioning and migration that have been reported for eukaryotes. The GFP-based assay is simple and of general applicability. It will be interesting to establish whether the novel type of dynamic behavior reported here, for higher plants, is observed in other eukaryotic organisms.

Original languageEnglish (US)
Pages (from-to)2733-2741
Number of pages9
JournalMolecular Biology of the Cell
Volume11
Issue number8
StatePublished - 2000

Fingerprint

Arabidopsis
Nuclear Pore
Nuclear Localization Signals
Membranes
Glucuronidase
Nuclear Envelope
Plant Cells
Eukaryotic Cells
Motion Pictures
Tubulin
Eukaryota
Fluorescence Microscopy
Confocal Microscopy
Actins
Proteins
Escherichia coli
Aequorea victoria green fluorescent protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Chytilova, E., Macas, J., Sliwinska, E., Rafelski, S. M., Lambert, G. M., & Galbraith, D. W. (2000). Nuclear dynamics in Arabidopsis thaliana. Molecular Biology of the Cell, 11(8), 2733-2741.

Nuclear dynamics in Arabidopsis thaliana. / Chytilova, E.; Macas, J.; Sliwinska, E.; Rafelski, S. M.; Lambert, G. M.; Galbraith, David W.

In: Molecular Biology of the Cell, Vol. 11, No. 8, 2000, p. 2733-2741.

Research output: Contribution to journalArticle

Chytilova, E, Macas, J, Sliwinska, E, Rafelski, SM, Lambert, GM & Galbraith, DW 2000, 'Nuclear dynamics in Arabidopsis thaliana', Molecular Biology of the Cell, vol. 11, no. 8, pp. 2733-2741.
Chytilova E, Macas J, Sliwinska E, Rafelski SM, Lambert GM, Galbraith DW. Nuclear dynamics in Arabidopsis thaliana. Molecular Biology of the Cell. 2000;11(8):2733-2741.
Chytilova, E. ; Macas, J. ; Sliwinska, E. ; Rafelski, S. M. ; Lambert, G. M. ; Galbraith, David W. / Nuclear dynamics in Arabidopsis thaliana. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 8. pp. 2733-2741.
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