Nucleated conformational conversion and the replication of conformational information by a prion determinant

Tricia R Serio, A. G. Cashikar, A. S. Kowal, G. J. Sawicki, J. J. Moslehi, L. Serpell, M. F. Arnsdorf, S. L. Lindquist

Research output: Contribution to journalArticle

734 Citations (Scopus)

Abstract

Prion proteins can serve as genetic elements by adopting distinct physical and functional states that are self-perpetuating and heritable. The critical region of one prion protein, Sup35, is initially unstructured in solution and then forms self-seeded amyloid fibers. We examined in vitro the mechanism by which this state is attained and replicated. Structurally fluid oligomeric complexes appear to be crucial intermediates in de novo amyloid nucleus formation. Rapid assembly ensues when these complexes conformationally convert upon association with nuclei. This model for replicating protein-based genetic information, nucleated conformational conversion, may be applicable to other protein assembly processes.

Original languageEnglish (US)
Pages (from-to)1317-1321
Number of pages5
JournalScience
Volume289
Issue number5483
DOIs
StatePublished - Aug 25 2000
Externally publishedYes

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Prions
Amyloid
Proteins
Prion Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Serio, T. R., Cashikar, A. G., Kowal, A. S., Sawicki, G. J., Moslehi, J. J., Serpell, L., ... Lindquist, S. L. (2000). Nucleated conformational conversion and the replication of conformational information by a prion determinant. Science, 289(5483), 1317-1321. https://doi.org/10.1126/science.289.5483.1317

Nucleated conformational conversion and the replication of conformational information by a prion determinant. / Serio, Tricia R; Cashikar, A. G.; Kowal, A. S.; Sawicki, G. J.; Moslehi, J. J.; Serpell, L.; Arnsdorf, M. F.; Lindquist, S. L.

In: Science, Vol. 289, No. 5483, 25.08.2000, p. 1317-1321.

Research output: Contribution to journalArticle

Serio, TR, Cashikar, AG, Kowal, AS, Sawicki, GJ, Moslehi, JJ, Serpell, L, Arnsdorf, MF & Lindquist, SL 2000, 'Nucleated conformational conversion and the replication of conformational information by a prion determinant', Science, vol. 289, no. 5483, pp. 1317-1321. https://doi.org/10.1126/science.289.5483.1317
Serio, Tricia R ; Cashikar, A. G. ; Kowal, A. S. ; Sawicki, G. J. ; Moslehi, J. J. ; Serpell, L. ; Arnsdorf, M. F. ; Lindquist, S. L. / Nucleated conformational conversion and the replication of conformational information by a prion determinant. In: Science. 2000 ; Vol. 289, No. 5483. pp. 1317-1321.
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