Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.

R. S. Kensinger, Robert J Collier, F. W. Bazer, C. A. Ducsay, H. N. Becker

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Changes in mammary gland histology, dry weights, nucleic acids and in vitro rates of substrate oxidation in incorporation into lipid were measured in mammary biopsies of three gilts each on d 30, 45, 60, 75, 90, 105 and 112 of pregnancy, and d 1 and 4 of lactation. Histological changes noted were progressive duct growth early in pregnancy followed by rapid lobulo-alveolar development between d 75 and 90 to complete mammogenesis. Colostrum and lipid were evident by d 105 with marked distension of alveolar lumina on d 112. Complete differentiation of the secretory process was apparent on the day of parturition. Concentrtion of dry, fat-free tissue (DFFT) and DNA changed little before d 60 but increased fourfold between d 75 and 90. No further increases in DFFT or DNA were noted. RNA concentrations paralleled DNA through d 90, after which they steadily increased. Rates of acetate and glucose oxidation increased transiently during midpregnancy then declined and remained low until initiation of lactogenesis. Substrate incorporation into lipid increased slightly at midpregnancy and again at d 105, after which it increased markedly. Collectively, results indicate that mammogenesis is complete by d 90, after which lactogenesis is initiated in a two-stage process. Metabolic rates expressed on a DNA basis indicated considerable rates of oxidation, but not of lipogenesis by proliferating mammary tissue. Preferential metabolism of acetate vs glucose near parturition suggests coordination of metabolism between the mammary gland and other maternal tissues.

Original languageEnglish (US)
Pages (from-to)1297-1308
Number of pages12
JournalJournal of Animal Science
Volume54
Issue number6
StatePublished - Jun 1982
Externally publishedYes

Fingerprint

milk synthesis
gilts
Nucleic Acids
nucleic acids
breasts
Breast
pregnancy
Pregnancy
DNA
mammary development
Human Mammary Glands
lipids
Lipids
oxidation
Acetates
mammary glands
Fats
Parturition
Glucose
Lipogenesis

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis. / Kensinger, R. S.; Collier, Robert J; Bazer, F. W.; Ducsay, C. A.; Becker, H. N.

In: Journal of Animal Science, Vol. 54, No. 6, 06.1982, p. 1297-1308.

Research output: Contribution to journalArticle

Kensinger, R. S. ; Collier, Robert J ; Bazer, F. W. ; Ducsay, C. A. ; Becker, H. N. / Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis. In: Journal of Animal Science. 1982 ; Vol. 54, No. 6. pp. 1297-1308.
@article{5441db36b8654b6db8533656e8d47918,
title = "Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.",
abstract = "Changes in mammary gland histology, dry weights, nucleic acids and in vitro rates of substrate oxidation in incorporation into lipid were measured in mammary biopsies of three gilts each on d 30, 45, 60, 75, 90, 105 and 112 of pregnancy, and d 1 and 4 of lactation. Histological changes noted were progressive duct growth early in pregnancy followed by rapid lobulo-alveolar development between d 75 and 90 to complete mammogenesis. Colostrum and lipid were evident by d 105 with marked distension of alveolar lumina on d 112. Complete differentiation of the secretory process was apparent on the day of parturition. Concentrtion of dry, fat-free tissue (DFFT) and DNA changed little before d 60 but increased fourfold between d 75 and 90. No further increases in DFFT or DNA were noted. RNA concentrations paralleled DNA through d 90, after which they steadily increased. Rates of acetate and glucose oxidation increased transiently during midpregnancy then declined and remained low until initiation of lactogenesis. Substrate incorporation into lipid increased slightly at midpregnancy and again at d 105, after which it increased markedly. Collectively, results indicate that mammogenesis is complete by d 90, after which lactogenesis is initiated in a two-stage process. Metabolic rates expressed on a DNA basis indicated considerable rates of oxidation, but not of lipogenesis by proliferating mammary tissue. Preferential metabolism of acetate vs glucose near parturition suggests coordination of metabolism between the mammary gland and other maternal tissues.",
author = "Kensinger, {R. S.} and Collier, {Robert J} and Bazer, {F. W.} and Ducsay, {C. A.} and Becker, {H. N.}",
year = "1982",
month = "6",
language = "English (US)",
volume = "54",
pages = "1297--1308",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "6",

}

TY - JOUR

T1 - Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.

AU - Kensinger, R. S.

AU - Collier, Robert J

AU - Bazer, F. W.

AU - Ducsay, C. A.

AU - Becker, H. N.

PY - 1982/6

Y1 - 1982/6

N2 - Changes in mammary gland histology, dry weights, nucleic acids and in vitro rates of substrate oxidation in incorporation into lipid were measured in mammary biopsies of three gilts each on d 30, 45, 60, 75, 90, 105 and 112 of pregnancy, and d 1 and 4 of lactation. Histological changes noted were progressive duct growth early in pregnancy followed by rapid lobulo-alveolar development between d 75 and 90 to complete mammogenesis. Colostrum and lipid were evident by d 105 with marked distension of alveolar lumina on d 112. Complete differentiation of the secretory process was apparent on the day of parturition. Concentrtion of dry, fat-free tissue (DFFT) and DNA changed little before d 60 but increased fourfold between d 75 and 90. No further increases in DFFT or DNA were noted. RNA concentrations paralleled DNA through d 90, after which they steadily increased. Rates of acetate and glucose oxidation increased transiently during midpregnancy then declined and remained low until initiation of lactogenesis. Substrate incorporation into lipid increased slightly at midpregnancy and again at d 105, after which it increased markedly. Collectively, results indicate that mammogenesis is complete by d 90, after which lactogenesis is initiated in a two-stage process. Metabolic rates expressed on a DNA basis indicated considerable rates of oxidation, but not of lipogenesis by proliferating mammary tissue. Preferential metabolism of acetate vs glucose near parturition suggests coordination of metabolism between the mammary gland and other maternal tissues.

AB - Changes in mammary gland histology, dry weights, nucleic acids and in vitro rates of substrate oxidation in incorporation into lipid were measured in mammary biopsies of three gilts each on d 30, 45, 60, 75, 90, 105 and 112 of pregnancy, and d 1 and 4 of lactation. Histological changes noted were progressive duct growth early in pregnancy followed by rapid lobulo-alveolar development between d 75 and 90 to complete mammogenesis. Colostrum and lipid were evident by d 105 with marked distension of alveolar lumina on d 112. Complete differentiation of the secretory process was apparent on the day of parturition. Concentrtion of dry, fat-free tissue (DFFT) and DNA changed little before d 60 but increased fourfold between d 75 and 90. No further increases in DFFT or DNA were noted. RNA concentrations paralleled DNA through d 90, after which they steadily increased. Rates of acetate and glucose oxidation increased transiently during midpregnancy then declined and remained low until initiation of lactogenesis. Substrate incorporation into lipid increased slightly at midpregnancy and again at d 105, after which it increased markedly. Collectively, results indicate that mammogenesis is complete by d 90, after which lactogenesis is initiated in a two-stage process. Metabolic rates expressed on a DNA basis indicated considerable rates of oxidation, but not of lipogenesis by proliferating mammary tissue. Preferential metabolism of acetate vs glucose near parturition suggests coordination of metabolism between the mammary gland and other maternal tissues.

UR - http://www.scopus.com/inward/record.url?scp=0020148335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020148335&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 1297

EP - 1308

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 6

ER -