Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies

Barbara Kutryb-Zajac, Ada H.Y. Yuen, Zain Khalpey, Paulina Zukowska, Ewa M. Slominska, Patricia M. Taylor, Steven Goldstein, Albert E. Heacox, Marialuisa Lavitrano, Adrian H. Chester, Magdi H. Yacoub, Ryszard T. Smolenski

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalJournal of cardiovascular translational research
Volume9
Issue number2
DOIs
StatePublished - Apr 1 2016

Keywords

  • (Max 10) heart valves
  • ATP
  • Adenosine
  • Nucleotide metabolism
  • Valve prostheses
  • Xenografts

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Pharmaceutical Science
  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)

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