Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies

Barbara Kutryb-Zajac, Ada H Y Yuen, Zain I Khalpey, Paulina Zukowska, Ewa M. Slominska, Patricia M. Taylor, Steven Goldstein, Albert E. Heacox, Marialuisa Lavitrano, Adrian H. Chester, Magdi H. Yacoub, Ryszard T. Smolenski

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalJournal of Cardiovascular Translational Research
Volume9
Issue number2
DOIs
StatePublished - Apr 1 2016

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Keywords

  • (Max 10) heart valves
  • Adenosine
  • ATP
  • Nucleotide metabolism
  • Valve prostheses
  • Xenografts

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Genetics(clinical)
  • Molecular Medicine
  • Pharmaceutical Science

Cite this

Kutryb-Zajac, B., Yuen, A. H. Y., Khalpey, Z. I., Zukowska, P., Slominska, E. M., Taylor, P. M., Goldstein, S., Heacox, A. E., Lavitrano, M., Chester, A. H., Yacoub, M. H., & Smolenski, R. T. (2016). Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies. Journal of Cardiovascular Translational Research, 9(2), 119-126. https://doi.org/10.1007/s12265-016-9672-6