nucleotide sequence analysis of a region upstream of the cholesterol oxidase-cytochrome p450 operon of streptomyces sp. SA-COO revealing repeating units coding for putative transmembrane and DNA-binding proteins

Istvan Molnar, Yoshikatsu Murooka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A 5.8-kb segment from the upstream region of the cholesterol oxidase (choA)-Cytochrome P450 (choP) operon of Streptomyces sp. SA-COO was sequenced. Computer assisted analysis of the sequence revealed four open reading frames (ORFs), whose deduced gene products could be classified into two groups. Cho-Orfl, the C-terminal segment of Cho-Orf3, and Cho-Orf4 were homologous to each other and showed similarities to the DNA-binding domains of bacterial response regulators of the UhpA subfamily, while a putative transmembrane protein, Cho-Orf2, and the N-terminal segment of Cho-Orf3 were homologous to each other but no homologies to known proteins were found. The genes coding for these putative proteins appeared to be organized as repeating units. Structural features of the nucleotide sequence and the homologies of the predicted gene products are discussed.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalJournal of Fermentation and Bioengineering
Volume76
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Cholesterol Oxidase
Cholesterol
DNA-Binding Proteins
Streptomyces
Operon
Nucleotides
Cytochrome P-450 Enzyme System
Sequence Analysis
DNA
Genes
Proteins
Sequence Homology
Open Reading Frames
carboxyl radical
Carrier Proteins
Oxidoreductases

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "nucleotide sequence analysis of a region upstream of the cholesterol oxidase-cytochrome p450 operon of streptomyces sp. SA-COO revealing repeating units coding for putative transmembrane and DNA-binding proteins",
abstract = "A 5.8-kb segment from the upstream region of the cholesterol oxidase (choA)-Cytochrome P450 (choP) operon of Streptomyces sp. SA-COO was sequenced. Computer assisted analysis of the sequence revealed four open reading frames (ORFs), whose deduced gene products could be classified into two groups. Cho-Orfl, the C-terminal segment of Cho-Orf3, and Cho-Orf4 were homologous to each other and showed similarities to the DNA-binding domains of bacterial response regulators of the UhpA subfamily, while a putative transmembrane protein, Cho-Orf2, and the N-terminal segment of Cho-Orf3 were homologous to each other but no homologies to known proteins were found. The genes coding for these putative proteins appeared to be organized as repeating units. Structural features of the nucleotide sequence and the homologies of the predicted gene products are discussed.",
author = "Istvan Molnar and Yoshikatsu Murooka",
year = "1993",
doi = "10.1016/0922-338X(93)90190-J",
language = "English (US)",
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pages = "257--264",
journal = "Journal of Bioscience and Bioengineering",
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T1 - nucleotide sequence analysis of a region upstream of the cholesterol oxidase-cytochrome p450 operon of streptomyces sp. SA-COO revealing repeating units coding for putative transmembrane and DNA-binding proteins

AU - Molnar, Istvan

AU - Murooka, Yoshikatsu

PY - 1993

Y1 - 1993

N2 - A 5.8-kb segment from the upstream region of the cholesterol oxidase (choA)-Cytochrome P450 (choP) operon of Streptomyces sp. SA-COO was sequenced. Computer assisted analysis of the sequence revealed four open reading frames (ORFs), whose deduced gene products could be classified into two groups. Cho-Orfl, the C-terminal segment of Cho-Orf3, and Cho-Orf4 were homologous to each other and showed similarities to the DNA-binding domains of bacterial response regulators of the UhpA subfamily, while a putative transmembrane protein, Cho-Orf2, and the N-terminal segment of Cho-Orf3 were homologous to each other but no homologies to known proteins were found. The genes coding for these putative proteins appeared to be organized as repeating units. Structural features of the nucleotide sequence and the homologies of the predicted gene products are discussed.

AB - A 5.8-kb segment from the upstream region of the cholesterol oxidase (choA)-Cytochrome P450 (choP) operon of Streptomyces sp. SA-COO was sequenced. Computer assisted analysis of the sequence revealed four open reading frames (ORFs), whose deduced gene products could be classified into two groups. Cho-Orfl, the C-terminal segment of Cho-Orf3, and Cho-Orf4 were homologous to each other and showed similarities to the DNA-binding domains of bacterial response regulators of the UhpA subfamily, while a putative transmembrane protein, Cho-Orf2, and the N-terminal segment of Cho-Orf3 were homologous to each other but no homologies to known proteins were found. The genes coding for these putative proteins appeared to be organized as repeating units. Structural features of the nucleotide sequence and the homologies of the predicted gene products are discussed.

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