Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens.

R. H. Kanemoto, A. T. Powell, D. E. Akiyoshi, D. A. Regier, R. A. Kerstetter, E. W. Nester, Martha C Hawes, M. P. Gordon

Research output: Contribution to journalArticle

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Abstract

Several loci on the tumor-inducing plasmid from Agrobacterium tumefaciens were transcriptionally activated in the presence of wounded plant tissue or extracts. The inducible virulence loci were required for efficient tumor formation. In contrast, the plant-inducible locus pinF was not observed to be absolutely essential for virulence. Mutants in pinF showed an attenuated virulence on a variety of dicotyledonous hosts, and this attenuation became more pronounced with decreasing numbers of bacterial cells in the inoculum. The DNA sequence of a 5.5-kilobase region which included the pinF locus from the octopine-type tumor-inducing plasmid A6 was determined. Four open reading frames consistent with the observed transcription of pinF were observed. Two of the open reading frames, pinF1 and pinF2, coded for polypeptides with relative molecular weights of 47,519 (pinF1) and 46,740 (pinF2). A comparison of the amino acid sequences of pinF1 and pinF2 indicated that they were similar to each other and to known polypeptide sequences for cytochrome P-450 enzymes.

Original languageEnglish (US)
Pages (from-to)2506-2512
Number of pages7
JournalJournal of Bacteriology
Volume171
Issue number5
StatePublished - May 1989
Externally publishedYes

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Agrobacterium tumefaciens
Sequence Analysis
Virulence
Open Reading Frames
Plasmids
Neoplasms
Peptides
Tissue Extracts
Cytochrome P-450 Enzyme System
Amino Acid Sequence
Cell Count
Molecular Weight

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Kanemoto, R. H., Powell, A. T., Akiyoshi, D. E., Regier, D. A., Kerstetter, R. A., Nester, E. W., ... Gordon, M. P. (1989). Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens. Journal of Bacteriology, 171(5), 2506-2512.

Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens. / Kanemoto, R. H.; Powell, A. T.; Akiyoshi, D. E.; Regier, D. A.; Kerstetter, R. A.; Nester, E. W.; Hawes, Martha C; Gordon, M. P.

In: Journal of Bacteriology, Vol. 171, No. 5, 05.1989, p. 2506-2512.

Research output: Contribution to journalArticle

Kanemoto, RH, Powell, AT, Akiyoshi, DE, Regier, DA, Kerstetter, RA, Nester, EW, Hawes, MC & Gordon, MP 1989, 'Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens.', Journal of Bacteriology, vol. 171, no. 5, pp. 2506-2512.
Kanemoto RH, Powell AT, Akiyoshi DE, Regier DA, Kerstetter RA, Nester EW et al. Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens. Journal of Bacteriology. 1989 May;171(5):2506-2512.
Kanemoto, R. H. ; Powell, A. T. ; Akiyoshi, D. E. ; Regier, D. A. ; Kerstetter, R. A. ; Nester, E. W. ; Hawes, Martha C ; Gordon, M. P. / Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens. In: Journal of Bacteriology. 1989 ; Vol. 171, No. 5. pp. 2506-2512.
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