Numerical simulation of long-range cell manipulation with AC electrokinetics by immersed boundary-lattice Boltzmann method

Qinlong Ren, Fanlong Meng, Cholik Chan

Research output: Contribution to journalConference article

Abstract

Alternating current (AC) electrokinetics have been widely used to manipulate bioparticles such as mammalian cell, bacteria, DNA, and protein among which the efficient cell patterning is essential for several biomedical and tissue engineering applications. Dielectrophoresis (DEP) is a well-known phenomenon that a force acts on the polarized particle in the non-uniform electric field, and it has been demonstrated as an effective, noninvasive, and non-destructive technique for cell trapping and separation. However, DEP force reduces dramatically with the decrease of electric field gradients which makes it ineffective for controlling the motion of cell in the region far away from the electrode edges. Fortunately, AC electrothermal (ACET) flow occurs when there exists electric field and temperature gradient caused by Joule heating or other external heat sources. The driving mechanism of ACET flow makes it important for pumping the physiological media of high conductivity under which circumstance the AC electroosmosis flow is weak. The drag force induced by ACET flow on the cell could transport it into the region near the electrode where the negative DEP force is significant. By balancing the DEP force, the drag force, and the gravitational force, the cell could be successfully suspended in the solution which is useful for cell patterning or other biomedical operations. In the current work, the immersed boundary-lattice Boltzmann method (IB-LBM) with GPU acceleration on a CUDA computational platform is used to accurately simulate the motion of cell under hybrid AC electrokinetic phenomena. The results indicate that a single cell could be successfully suspended at an equilibrium position due to the balanced ACET electrohydrodynamic force, negative DEP force, and gravitational force. However, the double cells confront a periodic motion around each other when the DEP force is not sufficient to overcome the effects of ACET flow and cell-cell interaction.

Original languageEnglish (US)
Pages (from-to)531-539
Number of pages9
JournalInternational Heat Transfer Conference
Volume2018-August
StatePublished - Jan 1 2018
Event16th International Heat Transfer Conference, IHTC 2018 - Beijing, China
Duration: Aug 10 2018Aug 15 2018

Fingerprint

electrokinetics
Electrophoresis
manipulators
alternating current
Computer simulation
cells
simulation
Electric fields
Drag
Electroosmosis
Electrohydrodynamics
Electrodes
Biomedical engineering
Joule heating
drag
Tissue engineering
electric fields
Thermal gradients
Bacteria
DNA

Keywords

  • AC electrothermal flow
  • Cell manipulation
  • Dielectrophoresis
  • Immersed boundary method
  • Lattice Boltzmann method

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Numerical simulation of long-range cell manipulation with AC electrokinetics by immersed boundary-lattice Boltzmann method. / Ren, Qinlong; Meng, Fanlong; Chan, Cholik.

In: International Heat Transfer Conference, Vol. 2018-August, 01.01.2018, p. 531-539.

Research output: Contribution to journalConference article

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