Nutritional mitigation of winter thermal stress in Nile tilapia by propolis-extract: Associated indicators of nutritional status, physiological responses and transcriptional response of delta-9-desaturase gene

Mohamed S. Hassaan, Ayman G. EL Nagar, Heba S. Salim, Kevin Fitzsimmons, Ehab R. El-Haroun

Research output: Contribution to journalArticle

Abstract

A feeding trial was conducted to investigate the ability of diet supplemented with propolis-extract (winter feed, WF) to assist Nile tilapia, Oreochromis niloticus in coping with winter thermal stress. Nile tilapia (average initial weight, 25.40 ± 0.84 g) was fed five isonitrogenous (303.20 g kg−1 crude protein) and isocaloric (19.44 MJ kg−1 gross energy) diets under thermal winter stress for 60 days. The diets contained five levels of propolis-extract (also known as bee glue): 0 (control), 1, 2, 3 and 4 g kg−1 diet. The range of water temperature throughout the experimental period was (16–19 °C). Results indicated that the survival rate was higher in fish fed with the diet supplemented with 4 g kg−1 of propolis-extract compared to the remaing diets. Weight gain and specific growth rate were linearly improved with increasing dietary propolis-extract inclusion (linear, P =.017; P =.051). Feed conversion ratio was significantly improved with the addition of dietary propolis (quadratic, P =.031). A linear response in hematocrit (linear, P =.001), hemoglobin (linear, P =.031), red blood cells count (linear, P =.041) and white blood cells count (linear, P =.003) of fish was also observed with the increasing level of propolis-extract. On its turn, significant quadratic decreases in alanine aminotransferase (P =.001), aspartate aminotransferase (P =.001), alkaline phosphatase (P =.002) and lactate dehydrogenase (P =.002) were detected with increasing dietary propolis-extract levels in the diet. There was a linear response in triglyceride and cholesterol of fish as the level of propolis-extract increased in the diet (linear, P =.071 and P =.003, respectively). The responses of serum cortisol and glucose in fish under cold stress tended to decrease in response to increasing dietary propolis-extract (respectively, quadratic, P =.001 and P =.012). The concentration levels of potassium was linearly (P =.001), and sodium quadraticly (P =.001) increased with dieatary proplis-extract. The expression of Δ 9D gene of fish under cold stress was upregulated (linearly, P =.001) with increasing dietary propolis-extract levels. To conclude, the optimum dietary inclusion level of propolis was estimated at 4 g kg−1 based on the maximization of the growth parameters and survival rate.

Original languageEnglish (US)
Article number734256
JournalAquaculture
Volume511
DOIs
StatePublished - Sep 15 2019

Fingerprint

propolis
thermal stress
nutritional status
physiological response
Oreochromis niloticus
mitigation
diet
winter
gene
extracts
genes
fish
blood
cold stress
indicator
hemoglobin
survival rate
phosphatase
bee
serum

Keywords

  • Cortisol
  • Hematology
  • Nile tilapia
  • Propolis-extract
  • Winter

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Nutritional mitigation of winter thermal stress in Nile tilapia by propolis-extract : Associated indicators of nutritional status, physiological responses and transcriptional response of delta-9-desaturase gene. / Hassaan, Mohamed S.; EL Nagar, Ayman G.; Salim, Heba S.; Fitzsimmons, Kevin; El-Haroun, Ehab R.

In: Aquaculture, Vol. 511, 734256, 15.09.2019.

Research output: Contribution to journalArticle

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abstract = "A feeding trial was conducted to investigate the ability of diet supplemented with propolis-extract (winter feed, WF) to assist Nile tilapia, Oreochromis niloticus in coping with winter thermal stress. Nile tilapia (average initial weight, 25.40 ± 0.84 g) was fed five isonitrogenous (303.20 g kg−1 crude protein) and isocaloric (19.44 MJ kg−1 gross energy) diets under thermal winter stress for 60 days. The diets contained five levels of propolis-extract (also known as bee glue): 0 (control), 1, 2, 3 and 4 g kg−1 diet. The range of water temperature throughout the experimental period was (16–19 °C). Results indicated that the survival rate was higher in fish fed with the diet supplemented with 4 g kg−1 of propolis-extract compared to the remaing diets. Weight gain and specific growth rate were linearly improved with increasing dietary propolis-extract inclusion (linear, P =.017; P =.051). Feed conversion ratio was significantly improved with the addition of dietary propolis (quadratic, P =.031). A linear response in hematocrit (linear, P =.001), hemoglobin (linear, P =.031), red blood cells count (linear, P =.041) and white blood cells count (linear, P =.003) of fish was also observed with the increasing level of propolis-extract. On its turn, significant quadratic decreases in alanine aminotransferase (P =.001), aspartate aminotransferase (P =.001), alkaline phosphatase (P =.002) and lactate dehydrogenase (P =.002) were detected with increasing dietary propolis-extract levels in the diet. There was a linear response in triglyceride and cholesterol of fish as the level of propolis-extract increased in the diet (linear, P =.071 and P =.003, respectively). The responses of serum cortisol and glucose in fish under cold stress tended to decrease in response to increasing dietary propolis-extract (respectively, quadratic, P =.001 and P =.012). The concentration levels of potassium was linearly (P =.001), and sodium quadraticly (P =.001) increased with dieatary proplis-extract. The expression of Δ 9D gene of fish under cold stress was upregulated (linearly, P =.001) with increasing dietary propolis-extract levels. To conclude, the optimum dietary inclusion level of propolis was estimated at 4 g kg−1 based on the maximization of the growth parameters and survival rate.",
keywords = "Cortisol, Hematology, Nile tilapia, Propolis-extract, Winter",
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T2 - Associated indicators of nutritional status, physiological responses and transcriptional response of delta-9-desaturase gene

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AU - EL Nagar, Ayman G.

AU - Salim, Heba S.

AU - Fitzsimmons, Kevin

AU - El-Haroun, Ehab R.

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AB - A feeding trial was conducted to investigate the ability of diet supplemented with propolis-extract (winter feed, WF) to assist Nile tilapia, Oreochromis niloticus in coping with winter thermal stress. Nile tilapia (average initial weight, 25.40 ± 0.84 g) was fed five isonitrogenous (303.20 g kg−1 crude protein) and isocaloric (19.44 MJ kg−1 gross energy) diets under thermal winter stress for 60 days. The diets contained five levels of propolis-extract (also known as bee glue): 0 (control), 1, 2, 3 and 4 g kg−1 diet. The range of water temperature throughout the experimental period was (16–19 °C). Results indicated that the survival rate was higher in fish fed with the diet supplemented with 4 g kg−1 of propolis-extract compared to the remaing diets. Weight gain and specific growth rate were linearly improved with increasing dietary propolis-extract inclusion (linear, P =.017; P =.051). Feed conversion ratio was significantly improved with the addition of dietary propolis (quadratic, P =.031). A linear response in hematocrit (linear, P =.001), hemoglobin (linear, P =.031), red blood cells count (linear, P =.041) and white blood cells count (linear, P =.003) of fish was also observed with the increasing level of propolis-extract. On its turn, significant quadratic decreases in alanine aminotransferase (P =.001), aspartate aminotransferase (P =.001), alkaline phosphatase (P =.002) and lactate dehydrogenase (P =.002) were detected with increasing dietary propolis-extract levels in the diet. There was a linear response in triglyceride and cholesterol of fish as the level of propolis-extract increased in the diet (linear, P =.071 and P =.003, respectively). The responses of serum cortisol and glucose in fish under cold stress tended to decrease in response to increasing dietary propolis-extract (respectively, quadratic, P =.001 and P =.012). The concentration levels of potassium was linearly (P =.001), and sodium quadraticly (P =.001) increased with dieatary proplis-extract. The expression of Δ 9D gene of fish under cold stress was upregulated (linearly, P =.001) with increasing dietary propolis-extract levels. To conclude, the optimum dietary inclusion level of propolis was estimated at 4 g kg−1 based on the maximization of the growth parameters and survival rate.

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KW - Hematology

KW - Nile tilapia

KW - Propolis-extract

KW - Winter

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