Objective assessment of multimodality optical coherence tomography and second-harmonic generation image quality of ex vivo mouse ovaries using human observers

Weston A. Welge, Andrew T. Demarco, Jennifer M. Watson, Photini S. Rice, Jennifer K. Barton, Matthew A. Kupinski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Ovarian cancer is particularly deadly because it is usually diagnosed after it has begun to spread. Transvaginal sonography (TVS) is the most common imaging screening technique. However, routine use of TVS has not reduced ovarian cancer mortality. The superior resolution of optical imaging techniques may make them attractive alternatives to TVS. We have previously identified features of ovarian cancer using optical coherence tomography (OCT) and secondharmonic generation (SHG) microscopy (with collagen as the targeted fluorophore). OCT provides a gross anatomical image of the ovary while SHG provides a closer look at a particular region. Knowing these anatomical features, we sought to investigate the diagnostic potential of OCT and SHG. We conducted a fully crossed, multi-reader, multi-case study using seven human observers. Each observer classified 44 ex vivo mouse ovaries as normal or abnormal from OCT, SHG, and simultaneous, co-registered OCT and SHG images and provided a confidence rating on a three-point ordinal scale. We determined the average receiver operating characteristic (ROC) curves, area under the ROC curves (AUC), and other quantitative figures of merit. The results show that OCT has diagnostic potential with an average AUC of 0.91 ± 0.03. The average AUC for SHG was less promising at 0.71 ± 0.06. Interestingly, the average AUC for simultaneous, co-registered OCT and SHG was not significantly different from OCT alone. This suggests that collagen may not be a useful fluorophore for ovarian cancer screening. The high performance of OCT warrants further investigation.

Original languageEnglish (US)
Title of host publicationDesign and Quality for Biomedical Technologies VII
PublisherSPIE
ISBN (Print)9780819498496
DOIs
StatePublished - Jan 1 2014
EventDesign and Quality for Biomedical Technologies VII - San Francisco, CA, United States
Duration: Feb 1 2014Feb 2 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8936
ISSN (Print)1605-7422

Other

OtherDesign and Quality for Biomedical Technologies VII
CountryUnited States
CitySan Francisco, CA
Period2/1/142/2/14

Keywords

  • Cancer screening
  • Image quality
  • Medical imaging
  • Multimodal imaging
  • Optical coherence tomography
  • Ovarian cancer
  • Receiver operating characteristic
  • Second harmonic generation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Welge, W. A., Demarco, A. T., Watson, J. M., Rice, P. S., Barton, J. K., & Kupinski, M. A. (2014). Objective assessment of multimodality optical coherence tomography and second-harmonic generation image quality of ex vivo mouse ovaries using human observers. In Design and Quality for Biomedical Technologies VII [893609] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8936). SPIE. https://doi.org/10.1117/12.2040520