Objective morphological quantification of microscopic images using a Fast Fourier Transform (FFT) analysis

Samuel E. Taylor, Tuoxin Cao, Pooja M. Talauliker, Jonathan Lifshitz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a numberof variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FFT intensity to the intensity of an orthogonal angle, giving a numerical value of the alignment of the microscopic structures. This allows for a more objective analysis than alternative approaches, which rely upon relative intensities.

Original languageEnglish (US)
Article number9.5
JournalCurrent Protocols in Essential Laboratory Techniques
Volume2013
Issue numberSUPPL.7
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Fourier Analysis
Fast Fourier transforms
Fluorescent Antibody Technique
Immunohistochemistry
Statistical methods

Keywords

  • Fast fourier transformation
  • Immunohistochemistry
  • Microscopy
  • Quantification

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Objective morphological quantification of microscopic images using a Fast Fourier Transform (FFT) analysis. / Taylor, Samuel E.; Cao, Tuoxin; Talauliker, Pooja M.; Lifshitz, Jonathan.

In: Current Protocols in Essential Laboratory Techniques, Vol. 2013, No. SUPPL.7, 9.5, 2013.

Research output: Contribution to journalArticle

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