Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Masaaki Kitajima, Andri T. Rachmadi, Brandon C. Iker, Eiji Haramoto, Ian L. Pepper, Charles P. Gerba

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 105 and 1.54 × 103 copies/liter in influent and effluent, respectively. The strains were characterized based on their 5’ untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.

Original languageEnglish (US)
Pages (from-to)1775-1779
Number of pages5
JournalArchives of virology
Volume160
Issue number7
DOIs
StatePublished - Jul 15 2015

Keywords

  • Cosavirus
  • Nested PCR
  • Phylogenetic analysis
  • Wastewater
  • qPCR

ASJC Scopus subject areas

  • Virology

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