Occurrence of Cryptosporidium oocysts in sewage effluents and selected surface waters.

M. S. Madore, J. B. Rose, Charles P Gerba, M. J. Arrowood, Charles R Sterling

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

An existing method for the detection of Cryptosporidium oocysts in water was modified to investigate oocyst prevalence in large volumes of water. Surface waters and sewage effluents were filtered, eluted from the filter, and concentrated using centrifugation. The resultant pellet was then homogenized, sonicated, and placed on a sucrose gradient to separate oocysts from the sediment. The uppermost gradient layer was then examined by immunofluorescence using a labeled monoclonal antibody. Using this technique, average numbers of oocysts detected in raw and treated sewage were 5.18 X 10(3) and 1.30 X 10(3)/L, respectively. Filtered sewage effluents had significantly lower numbers of oocysts (10.0/L). These data show that sand filtration may reduce the concentrations of this parasite in waste waters. Highly variable oocyst numbers were encountered in surface waters. Since Cryptosporidium oocysts are frequently present in environmental waters, they could be responsible for waterborne outbreaks of disease.

Original languageEnglish (US)
Pages (from-to)702-705
Number of pages4
JournalJournal of Parasitology
Volume73
Issue number4
StatePublished - Aug 1987
Externally publishedYes

Fingerprint

sewage effluent
Cryptosporidium
Oocysts
Sewage
oocysts
surface water
sewage
effluent
Water
sucrose
water
antibody
parasite
filter
sand
sediment
Waste Water
Centrifugation
centrifugation
fluorescent antibody technique

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology
  • Parasitology

Cite this

Occurrence of Cryptosporidium oocysts in sewage effluents and selected surface waters. / Madore, M. S.; Rose, J. B.; Gerba, Charles P; Arrowood, M. J.; Sterling, Charles R.

In: Journal of Parasitology, Vol. 73, No. 4, 08.1987, p. 702-705.

Research output: Contribution to journalArticle

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