Abstract
As new environments are studied, viruses consistently emerge as important and prominent players in natural and man-made ecosystems. However, much of what we know is built both upon the foundation of the culturable minority and using methods that are often insufficiently ground-truthed. Here, we review the modern culture-independent viral metagenomic sample-to-sequence pipeline and how next-generation sequencing techniques are drastically altering our ability to systematically and rigorously evaluate them. Together, a series of studies quantitatively evaluate existing and new methods that allow-even for ultra-low DNA samples-the generation of replicable, near-quantitative datasets that maximize inter-comparability and biological inference.
Original language | English (US) |
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Pages (from-to) | 181-186 |
Number of pages | 6 |
Journal | Virology |
Volume | 434 |
Issue number | 2 |
DOIs | |
State | Published - Dec 20 2012 |
Keywords
- Environmental virology
- Metagenomics
- Next-generation sequencing
- Ocean viruses
- Viral concentration
- Viral ecology
- Viral purification
ASJC Scopus subject areas
- Virology