On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array

Xiangjun Zheng, Linan Jiang, Joyce Schroeder, Marilyn Marron, Maria E Iannone, Alison T Stopeck, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1 to 1:100,000. The dependence of the device performance, particularly its sensitivity and specificity in isolating target cells, on the applied flow shear rate is characterized. The results demonstrate capture of circulating tumor cells from mixtures with high sensitivity and high specificity. The multichannel array devices allow testing of samples within a shorter time, which is critical for high throughput applications.

Original languageEnglish (US)
Title of host publicationIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Pages87-91
Number of pages5
DOIs
StatePublished - 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: Nov 4 2012Nov 7 2012

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period11/4/1211/7/12

Fingerprint

Circulating Neoplastic Cells
Binary mixtures
Microchannels
Antibodies
Limit of Detection
Tumors
Cells
Shear deformation
Throughput
Sensitivity and Specificity
Equipment and Supplies
Testing
Epithelial Cell Adhesion Molecule

Keywords

  • Bio-functional microchannel array
  • Circulating tumor cells
  • High throughput
  • Minimum detection limit

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Zheng, X., Jiang, L., Schroeder, J., Marron, M., Iannone, M. E., Stopeck, A. T., & Zohar, Y. (2012). On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. In IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED (pp. 87-91). [6509115] https://doi.org/10.1109/NANOMED.2012.6509115

On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. / Zheng, Xiangjun; Jiang, Linan; Schroeder, Joyce; Marron, Marilyn; Iannone, Maria E; Stopeck, Alison T; Zohar, Yitshak.

IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. p. 87-91 6509115.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zheng, X, Jiang, L, Schroeder, J, Marron, M, Iannone, ME, Stopeck, AT & Zohar, Y 2012, On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. in IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED., 6509115, pp. 87-91, 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012, Bangkok, Thailand, 11/4/12. https://doi.org/10.1109/NANOMED.2012.6509115
Zheng X, Jiang L, Schroeder J, Marron M, Iannone ME, Stopeck AT et al. On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. In IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. p. 87-91. 6509115 https://doi.org/10.1109/NANOMED.2012.6509115
Zheng, Xiangjun ; Jiang, Linan ; Schroeder, Joyce ; Marron, Marilyn ; Iannone, Maria E ; Stopeck, Alison T ; Zohar, Yitshak. / On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. pp. 87-91
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abstract = "The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1 to 1:100,000. The dependence of the device performance, particularly its sensitivity and specificity in isolating target cells, on the applied flow shear rate is characterized. The results demonstrate capture of circulating tumor cells from mixtures with high sensitivity and high specificity. The multichannel array devices allow testing of samples within a shorter time, which is critical for high throughput applications.",
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