On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array

Xiangjun Zheng, Linan Jiang, Joyce A. Schroeder, Marilyn Marron, Maria Iannone, Alison T. Stopeck, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1 to 1:100,000. The dependence of the device performance, particularly its sensitivity and specificity in isolating target cells, on the applied flow shear rate is characterized. The results demonstrate capture of circulating tumor cells from mixtures with high sensitivity and high specificity. The multichannel array devices allow testing of samples within a shorter time, which is critical for high throughput applications.

Original languageEnglish (US)
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages87-91
Number of pages5
DOIs
StatePublished - Dec 1 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: Nov 4 2012Nov 7 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period11/4/1211/7/12

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Keywords

  • Bio-functional microchannel array
  • Circulating tumor cells
  • High throughput
  • Minimum detection limit

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Zheng, X., Jiang, L., Schroeder, J. A., Marron, M., Iannone, M., Stopeck, A. T., & Zohar, Y. (2012). On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 87-91). [6509115] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509115