Abstract
A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2355-2359 |
| Number of pages | 5 |
| Journal | Journal of Proteome Research |
| Volume | 4 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2005 |
| Externally published | Yes |
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Keywords
- Elastin-like polypeptide
- Glyco-affinity precipitation
- Glycoconjugate
- Protein purification
ASJC Scopus subject areas
- Genetics
- Biotechnology
- Biochemistry
Cite this
One-pot glyco-affinity precipitation purification for enhanced proteomics : The flexible alignment of solution-phase capture/release and solid-phase separation. / Sun, Xue Long; Haller, Carolyn A.; Wu, Xiaoyi; Conticello, Vincent P.; Chaikof, Elliot L.
In: Journal of Proteome Research, Vol. 4, No. 6, 11.2005, p. 2355-2359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - One-pot glyco-affinity precipitation purification for enhanced proteomics
T2 - The flexible alignment of solution-phase capture/release and solid-phase separation
AU - Sun, Xue Long
AU - Haller, Carolyn A.
AU - Wu, Xiaoyi
AU - Conticello, Vincent P.
AU - Chaikof, Elliot L.
PY - 2005/11
Y1 - 2005/11
N2 - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.
AB - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.
KW - Elastin-like polypeptide
KW - Glyco-affinity precipitation
KW - Glycoconjugate
KW - Protein purification
UR - http://www.scopus.com/inward/record.url?scp=29144478731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29144478731&partnerID=8YFLogxK
U2 - 10.1021/pr050245g
DO - 10.1021/pr050245g
M3 - Article
C2 - 16335985
AN - SCOPUS:29144478731
VL - 4
SP - 2355
EP - 2359
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 6
ER -