One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun, Carolyn A. Haller, Xiaoyi Wu, Vincent P. Conticello, Elliot L. Chaikof

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

Original languageEnglish (US)
Pages (from-to)2355-2359
Number of pages5
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Phase separation
Proteomics
Purification
Polymers
Carbohydrates
Galectins
Elastin
Transition Temperature
Cell Separation
Differential Scanning Calorimetry
Pathogens
Lactose
Galactose
Lectins
Nuclear magnetic resonance spectroscopy
Differential scanning calorimetry
Magnetic Resonance Spectroscopy
Copolymers
Ligands
Peptides

Keywords

  • Elastin-like polypeptide
  • Glyco-affinity precipitation
  • Glycoconjugate
  • Protein purification

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

One-pot glyco-affinity precipitation purification for enhanced proteomics : The flexible alignment of solution-phase capture/release and solid-phase separation. / Sun, Xue Long; Haller, Carolyn A.; Wu, Xiaoyi; Conticello, Vincent P.; Chaikof, Elliot L.

In: Journal of Proteome Research, Vol. 4, No. 6, 11.2005, p. 2355-2359.

Research output: Contribution to journalArticle

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