One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun; Carolyn A. Haller; Xiao Yi Wu; Vincent P. Conticello; Elliot L. Chaikof

doi:10.1021/pr050245g

One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun, Carolyn A. Haller, Xiao Yi Wu, Vincent P. Conticello, Elliot L. Chaikof

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and ¹H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

Original language	English (US)
Pages (from-to)	2355-2359
Number of pages	5
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr050245g
State	Published - Nov 1 2005
Externally published	Yes

Keywords

Elastin-like polypeptide
Glyco-affinity precipitation
Glycoconjugate
Protein purification

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr050245g

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One-pot glyco-affinity precipitation purification for enhanced proteomics : The flexible alignment of solution-phase capture/release and solid-phase separation. / Sun, Xue Long; Haller, Carolyn A.; Wu, Xiao Yi; Conticello, Vincent P.; Chaikof, Elliot L.

In: Journal of Proteome Research, Vol. 4, No. 6, 01.11.2005, p. 2355-2359.

Research output: Contribution to journal › Article › peer-review

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abstract = "A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.",

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AU - Haller, Carolyn A.

AU - Wu, Xiao Yi

AU - Conticello, Vincent P.

AU - Chaikof, Elliot L.

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AB - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

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One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Abstract

Keywords

ASJC Scopus subject areas

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