One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun, Carolyn A. Haller, Xiao Yi Wu, Vincent P. Conticello, Elliot L. Chaikof

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

Original languageEnglish (US)
Pages (from-to)2355-2359
Number of pages5
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Keywords

  • Elastin-like polypeptide
  • Glyco-affinity precipitation
  • Glycoconjugate
  • Protein purification

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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