Online photolytic optical gating of caged fluorophores in capillary zone electrophoresis utilizing an ultraviolet light-emitting diode

Elyssia S. Gallagher, Troy J. Comi, Kevin L. Braun, Craig A. Aspinwall

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Photolytic optical gating (POG) facilitates rapid, on-line and highly sensitive analyses, though POG utilizes UV lasers for sample injection. We present a low-cost, more portable alternative, employing an ultraviolet light-emitting diode (UV-LED) array to inject caged fluorescent dyes via photolysis. Utilizing the UV-LED array, labeled amino acids were injected with nanomolar limits of detection (270 ± 30 nM and 250 ± 30 nM for arginine and citrulline, respectively). When normalized for the difference in light intensity, the UV-LED array provides comparable sensitivity to POG utilizing UV lasers. Additionally, the UV-LED array yielded sufficient beam quality and stability to facilitate coupling with a Hadamard transform, resulting in increased sensitivity. This work shows, for the first time, the use of an UV-LED for online POG with comparable sensitivity to conventional laser sources but at a lower cost.

Original languageEnglish (US)
Pages (from-to)2903-2910
Number of pages8
JournalELECTROPHORESIS
Volume33
Issue number18
DOIs
StatePublished - Sep 2012

Keywords

  • Capillary zone electrophoresis
  • Hadamard transform
  • Photolytic optical gating
  • Ultraviolet light-emitting diode

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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