Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm

Junpeng Zhan, Guosheng Li, Choong Hwan Ryu, Chuang Ma, Shanshan Zhang, Alan Lloyd, Brenda G. Hunter, Brian A. Larkins, Gary N. Drews, Xiangfeng Wang, Ramin Yadegari

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Development of the cereal endosperm involves cell differentiation processes that enable nutrient uptake from the maternal plant, accumulation of storage products, and their utilization during germination. However, little is known about the regulatory mechanisms that link cell differentiation processes with those controlling storage product synthesis and deposition, including the activation of zein genes by the maize (Zea mays) bZIP transcription factor Opaque-2 (O2). Here, we mapped in vivo binding sites of O2 in B73 endosperm and compared the results with genes differentially expressed in B73 and B73o2 We identified 186 putative direct O2 targets and 1677 indirect targets, encoding a broad set of gene functionalities. Examination of the temporal expression patterns of O2 targets revealed at least two distinct modes of O2-mediated gene activation. Two O2-activated genes, bZIP17 and NAKED ENDOSPERM2 (NKD2), encode transcription factors, which can in turn coactivate other O2 network genes with O2. NKD2 (with its paralog NKD1) was previously shown to be involved in regulation of aleurone development. Collectively, our results provide insights into the complexity of the O2-regulated network and its role in regulation of endosperm cell differentiation and function.

Original languageEnglish (US)
Pages (from-to)2425-2446
Number of pages22
JournalThe Plant cell
Volume30
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

Endosperm
Gene Regulatory Networks
cell differentiation
endosperm
Zea mays
Cell Differentiation
Transcriptional Activation
corn
Zein
Basic-Leucine Zipper Transcription Factors
Genes
genes
Germination
zein
gene activation
Transcription Factors
Binding Sites
Mothers
nutrient uptake
binding sites

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm. / Zhan, Junpeng; Li, Guosheng; Ryu, Choong Hwan; Ma, Chuang; Zhang, Shanshan; Lloyd, Alan; Hunter, Brenda G.; Larkins, Brian A.; Drews, Gary N.; Wang, Xiangfeng; Yadegari, Ramin.

In: The Plant cell, Vol. 30, No. 10, 01.10.2018, p. 2425-2446.

Research output: Contribution to journalArticle

Zhan, J, Li, G, Ryu, CH, Ma, C, Zhang, S, Lloyd, A, Hunter, BG, Larkins, BA, Drews, GN, Wang, X & Yadegari, R 2018, 'Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm', The Plant cell, vol. 30, no. 10, pp. 2425-2446. https://doi.org/10.1105/tpc.18.00392
Zhan, Junpeng ; Li, Guosheng ; Ryu, Choong Hwan ; Ma, Chuang ; Zhang, Shanshan ; Lloyd, Alan ; Hunter, Brenda G. ; Larkins, Brian A. ; Drews, Gary N. ; Wang, Xiangfeng ; Yadegari, Ramin. / Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm. In: The Plant cell. 2018 ; Vol. 30, No. 10. pp. 2425-2446.
@article{dd252289eb194ee8acf47d7c4ee02156,
title = "Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm",
abstract = "Development of the cereal endosperm involves cell differentiation processes that enable nutrient uptake from the maternal plant, accumulation of storage products, and their utilization during germination. However, little is known about the regulatory mechanisms that link cell differentiation processes with those controlling storage product synthesis and deposition, including the activation of zein genes by the maize (Zea mays) bZIP transcription factor Opaque-2 (O2). Here, we mapped in vivo binding sites of O2 in B73 endosperm and compared the results with genes differentially expressed in B73 and B73o2 We identified 186 putative direct O2 targets and 1677 indirect targets, encoding a broad set of gene functionalities. Examination of the temporal expression patterns of O2 targets revealed at least two distinct modes of O2-mediated gene activation. Two O2-activated genes, bZIP17 and NAKED ENDOSPERM2 (NKD2), encode transcription factors, which can in turn coactivate other O2 network genes with O2. NKD2 (with its paralog NKD1) was previously shown to be involved in regulation of aleurone development. Collectively, our results provide insights into the complexity of the O2-regulated network and its role in regulation of endosperm cell differentiation and function.",
author = "Junpeng Zhan and Guosheng Li and Ryu, {Choong Hwan} and Chuang Ma and Shanshan Zhang and Alan Lloyd and Hunter, {Brenda G.} and Larkins, {Brian A.} and Drews, {Gary N.} and Xiangfeng Wang and Ramin Yadegari",
year = "2018",
month = "10",
day = "1",
doi = "10.1105/tpc.18.00392",
language = "English (US)",
volume = "30",
pages = "2425--2446",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "10",

}

TY - JOUR

T1 - Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm

AU - Zhan, Junpeng

AU - Li, Guosheng

AU - Ryu, Choong Hwan

AU - Ma, Chuang

AU - Zhang, Shanshan

AU - Lloyd, Alan

AU - Hunter, Brenda G.

AU - Larkins, Brian A.

AU - Drews, Gary N.

AU - Wang, Xiangfeng

AU - Yadegari, Ramin

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Development of the cereal endosperm involves cell differentiation processes that enable nutrient uptake from the maternal plant, accumulation of storage products, and their utilization during germination. However, little is known about the regulatory mechanisms that link cell differentiation processes with those controlling storage product synthesis and deposition, including the activation of zein genes by the maize (Zea mays) bZIP transcription factor Opaque-2 (O2). Here, we mapped in vivo binding sites of O2 in B73 endosperm and compared the results with genes differentially expressed in B73 and B73o2 We identified 186 putative direct O2 targets and 1677 indirect targets, encoding a broad set of gene functionalities. Examination of the temporal expression patterns of O2 targets revealed at least two distinct modes of O2-mediated gene activation. Two O2-activated genes, bZIP17 and NAKED ENDOSPERM2 (NKD2), encode transcription factors, which can in turn coactivate other O2 network genes with O2. NKD2 (with its paralog NKD1) was previously shown to be involved in regulation of aleurone development. Collectively, our results provide insights into the complexity of the O2-regulated network and its role in regulation of endosperm cell differentiation and function.

AB - Development of the cereal endosperm involves cell differentiation processes that enable nutrient uptake from the maternal plant, accumulation of storage products, and their utilization during germination. However, little is known about the regulatory mechanisms that link cell differentiation processes with those controlling storage product synthesis and deposition, including the activation of zein genes by the maize (Zea mays) bZIP transcription factor Opaque-2 (O2). Here, we mapped in vivo binding sites of O2 in B73 endosperm and compared the results with genes differentially expressed in B73 and B73o2 We identified 186 putative direct O2 targets and 1677 indirect targets, encoding a broad set of gene functionalities. Examination of the temporal expression patterns of O2 targets revealed at least two distinct modes of O2-mediated gene activation. Two O2-activated genes, bZIP17 and NAKED ENDOSPERM2 (NKD2), encode transcription factors, which can in turn coactivate other O2 network genes with O2. NKD2 (with its paralog NKD1) was previously shown to be involved in regulation of aleurone development. Collectively, our results provide insights into the complexity of the O2-regulated network and its role in regulation of endosperm cell differentiation and function.

UR - http://www.scopus.com/inward/record.url?scp=85056306636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056306636&partnerID=8YFLogxK

U2 - 10.1105/tpc.18.00392

DO - 10.1105/tpc.18.00392

M3 - Article

C2 - 30262552

AN - SCOPUS:85056306636

VL - 30

SP - 2425

EP - 2446

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 10

ER -