Opioid agonist and antagonist antinociceptive properties of [D-Ala2,Leu5,Cys6]enkephalin: Selective actions at the delta(noncomplexed) site

Q. Jiang, W. D. Bowen, H. I. Mosberg, R. B. Rothman, Frank Porreca

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The present study used the irreversibly binding enkephalin analog, [D-Ala2,Leu5,Cys6]enkephalin (DALCE) in an effort to determine whether selective agonist and antagonist properties could be demonstrated at hypothesized types of opioid delta receptors previously termed the delta(noncomplexed) and the delta(complexed) sites. These putative subtypes of delta receptors have been functionally distinguished on the basis of involvement (i.e., delta(complexed)) in the modulation of mu-mediated effects such as antinociception. Intracerebroventricular administration of DALCE or the reference delta and mu agonists, [D-Pen2,D-Pen5]enkephalin (DPDPE) and morphine, to mice all produced antinociception in the warm-water tail-flick test in a dose- and time-related manner. Maximal effects with DALCE were seen at +10 min and significant antinociception could be detected for approximately 1 hr; DALCE was 3- and 90-fold more potent than i.c.v. morphine and DPDPE, respectively. The antinociceptive effects of i.c.v. DALCE and DPDPE, but not those of morphine, were antagonized by the selective delta antagonist, N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH, suggesting that the antinociception associated with the peptides was mediated through a delta receptor. DALCE pre-treatment up to 24 hr before testing, a time at which this compound did not produce antinociception, significantly blocked the i.c.v. DPDPE antinociceptive effect as well as that of DALCE itself, but not that of morphine, suggesting long-lasting DALCE antagonism at a delta receptor. Modulation of morphine antinociception was demonstrated with subeffective doses of i.c.v. DPDPE or [Met5]enkephalin, but not with subeffective doses of i.c.v. DALCE. In addition to a lack of modulation of morphine antinociception after acute administration, i.c.v. DALCE (at -24 hr) did not directly antagonize the antinociceptive actions of morphine or block the modulation of morphine by DPDPE or [Met5]enkephalin. These data suggest that i.c.v. DALCE given acutely produces direct antinociceptive actions through a supraspinal delta receptor, and additionally, DALCE subsequently acts as a long-lasting delta antagonist. However, unlike the actions of DPDPE or [Met5]enkephalin, neither the direct delta agonist or antagonist actions of DALCE are associated with indirect modulation of morphine antinociception. These findings provide further support for the concept of a functional opioid mu-delta receptor complex and support the existence of subtypes of opioid delta receptors that may be distinguished on the basis of their modulation of mu agonist actions (i.e., delta(complexed) and delta(noncomplexed) receptors); thus, DALCE appears to selectively interact with the delta(noncomplexed) receptor.

Original languageEnglish (US)
Pages (from-to)636-641
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume255
Issue number2
StatePublished - 1990

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Narcotic Antagonists
Enkephalins
D-Penicillamine (2,5)-Enkephalin
delta Opioid Receptor
Morphine
mu Opioid Receptor

ASJC Scopus subject areas

  • Pharmacology

Cite this

Opioid agonist and antagonist antinociceptive properties of [D-Ala2,Leu5,Cys6]enkephalin : Selective actions at the delta(noncomplexed) site. / Jiang, Q.; Bowen, W. D.; Mosberg, H. I.; Rothman, R. B.; Porreca, Frank.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 255, No. 2, 1990, p. 636-641.

Research output: Contribution to journalArticle

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abstract = "The present study used the irreversibly binding enkephalin analog, [D-Ala2,Leu5,Cys6]enkephalin (DALCE) in an effort to determine whether selective agonist and antagonist properties could be demonstrated at hypothesized types of opioid delta receptors previously termed the delta(noncomplexed) and the delta(complexed) sites. These putative subtypes of delta receptors have been functionally distinguished on the basis of involvement (i.e., delta(complexed)) in the modulation of mu-mediated effects such as antinociception. Intracerebroventricular administration of DALCE or the reference delta and mu agonists, [D-Pen2,D-Pen5]enkephalin (DPDPE) and morphine, to mice all produced antinociception in the warm-water tail-flick test in a dose- and time-related manner. Maximal effects with DALCE were seen at +10 min and significant antinociception could be detected for approximately 1 hr; DALCE was 3- and 90-fold more potent than i.c.v. morphine and DPDPE, respectively. The antinociceptive effects of i.c.v. DALCE and DPDPE, but not those of morphine, were antagonized by the selective delta antagonist, N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH, suggesting that the antinociception associated with the peptides was mediated through a delta receptor. DALCE pre-treatment up to 24 hr before testing, a time at which this compound did not produce antinociception, significantly blocked the i.c.v. DPDPE antinociceptive effect as well as that of DALCE itself, but not that of morphine, suggesting long-lasting DALCE antagonism at a delta receptor. Modulation of morphine antinociception was demonstrated with subeffective doses of i.c.v. DPDPE or [Met5]enkephalin, but not with subeffective doses of i.c.v. DALCE. In addition to a lack of modulation of morphine antinociception after acute administration, i.c.v. DALCE (at -24 hr) did not directly antagonize the antinociceptive actions of morphine or block the modulation of morphine by DPDPE or [Met5]enkephalin. These data suggest that i.c.v. DALCE given acutely produces direct antinociceptive actions through a supraspinal delta receptor, and additionally, DALCE subsequently acts as a long-lasting delta antagonist. However, unlike the actions of DPDPE or [Met5]enkephalin, neither the direct delta agonist or antagonist actions of DALCE are associated with indirect modulation of morphine antinociception. These findings provide further support for the concept of a functional opioid mu-delta receptor complex and support the existence of subtypes of opioid delta receptors that may be distinguished on the basis of their modulation of mu agonist actions (i.e., delta(complexed) and delta(noncomplexed) receptors); thus, DALCE appears to selectively interact with the delta(noncomplexed) receptor.",
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N2 - The present study used the irreversibly binding enkephalin analog, [D-Ala2,Leu5,Cys6]enkephalin (DALCE) in an effort to determine whether selective agonist and antagonist properties could be demonstrated at hypothesized types of opioid delta receptors previously termed the delta(noncomplexed) and the delta(complexed) sites. These putative subtypes of delta receptors have been functionally distinguished on the basis of involvement (i.e., delta(complexed)) in the modulation of mu-mediated effects such as antinociception. Intracerebroventricular administration of DALCE or the reference delta and mu agonists, [D-Pen2,D-Pen5]enkephalin (DPDPE) and morphine, to mice all produced antinociception in the warm-water tail-flick test in a dose- and time-related manner. Maximal effects with DALCE were seen at +10 min and significant antinociception could be detected for approximately 1 hr; DALCE was 3- and 90-fold more potent than i.c.v. morphine and DPDPE, respectively. The antinociceptive effects of i.c.v. DALCE and DPDPE, but not those of morphine, were antagonized by the selective delta antagonist, N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH, suggesting that the antinociception associated with the peptides was mediated through a delta receptor. DALCE pre-treatment up to 24 hr before testing, a time at which this compound did not produce antinociception, significantly blocked the i.c.v. DPDPE antinociceptive effect as well as that of DALCE itself, but not that of morphine, suggesting long-lasting DALCE antagonism at a delta receptor. Modulation of morphine antinociception was demonstrated with subeffective doses of i.c.v. DPDPE or [Met5]enkephalin, but not with subeffective doses of i.c.v. DALCE. In addition to a lack of modulation of morphine antinociception after acute administration, i.c.v. DALCE (at -24 hr) did not directly antagonize the antinociceptive actions of morphine or block the modulation of morphine by DPDPE or [Met5]enkephalin. These data suggest that i.c.v. DALCE given acutely produces direct antinociceptive actions through a supraspinal delta receptor, and additionally, DALCE subsequently acts as a long-lasting delta antagonist. However, unlike the actions of DPDPE or [Met5]enkephalin, neither the direct delta agonist or antagonist actions of DALCE are associated with indirect modulation of morphine antinociception. These findings provide further support for the concept of a functional opioid mu-delta receptor complex and support the existence of subtypes of opioid delta receptors that may be distinguished on the basis of their modulation of mu agonist actions (i.e., delta(complexed) and delta(noncomplexed) receptors); thus, DALCE appears to selectively interact with the delta(noncomplexed) receptor.

AB - The present study used the irreversibly binding enkephalin analog, [D-Ala2,Leu5,Cys6]enkephalin (DALCE) in an effort to determine whether selective agonist and antagonist properties could be demonstrated at hypothesized types of opioid delta receptors previously termed the delta(noncomplexed) and the delta(complexed) sites. These putative subtypes of delta receptors have been functionally distinguished on the basis of involvement (i.e., delta(complexed)) in the modulation of mu-mediated effects such as antinociception. Intracerebroventricular administration of DALCE or the reference delta and mu agonists, [D-Pen2,D-Pen5]enkephalin (DPDPE) and morphine, to mice all produced antinociception in the warm-water tail-flick test in a dose- and time-related manner. Maximal effects with DALCE were seen at +10 min and significant antinociception could be detected for approximately 1 hr; DALCE was 3- and 90-fold more potent than i.c.v. morphine and DPDPE, respectively. The antinociceptive effects of i.c.v. DALCE and DPDPE, but not those of morphine, were antagonized by the selective delta antagonist, N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH, suggesting that the antinociception associated with the peptides was mediated through a delta receptor. DALCE pre-treatment up to 24 hr before testing, a time at which this compound did not produce antinociception, significantly blocked the i.c.v. DPDPE antinociceptive effect as well as that of DALCE itself, but not that of morphine, suggesting long-lasting DALCE antagonism at a delta receptor. Modulation of morphine antinociception was demonstrated with subeffective doses of i.c.v. DPDPE or [Met5]enkephalin, but not with subeffective doses of i.c.v. DALCE. In addition to a lack of modulation of morphine antinociception after acute administration, i.c.v. DALCE (at -24 hr) did not directly antagonize the antinociceptive actions of morphine or block the modulation of morphine by DPDPE or [Met5]enkephalin. These data suggest that i.c.v. DALCE given acutely produces direct antinociceptive actions through a supraspinal delta receptor, and additionally, DALCE subsequently acts as a long-lasting delta antagonist. However, unlike the actions of DPDPE or [Met5]enkephalin, neither the direct delta agonist or antagonist actions of DALCE are associated with indirect modulation of morphine antinociception. These findings provide further support for the concept of a functional opioid mu-delta receptor complex and support the existence of subtypes of opioid delta receptors that may be distinguished on the basis of their modulation of mu agonist actions (i.e., delta(complexed) and delta(noncomplexed) receptors); thus, DALCE appears to selectively interact with the delta(noncomplexed) receptor.

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