Opioid peptide receptor studies. 4. Antisense oligodeoxynucleotide to the delta opioid receptor delineates opioid receptor subtypes

Xian Yuan Cha, Heng Xu, Qing Ni, John S. Partilla, Kenner C. Rice, Dorota Matecka, Silvia N. Calderon, Frank Porreca, Josephine Lai, Richard B. Rothman

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Prior work in our laboratory has identified putative subtypes of δ (δcx-1, δcx-2, δncx-1, δncx-2) and κ22a and κ2b) receptors. Previous studies showed that chronic (three day) i.c.v. administration of antisense oligodeoxynucleotide to the cloned δ opioid receptor selectively decreased [3H][d-Ala2,d-Leu5]enkephalin binding to the δncx site, not the δcx-2 site. The present study extends this work by demonstrating that δ antisense DNA selectively affects the δncx-2 site sparing the other putative δ receptor subtypes and κ2 receptor subtypes. This selectivity is not due to anatomically specific effects of δ antisense DNA since autoradiograms show that δ binding is reduced in all regions of the brain after chronic i.c.v. administration of δ antisense DNA. These data strongly suggest that the δcx-1, δcx-2, δncx-1, κ2a and κ2b binding sites are different proteins than the δncx-2 binding site, which, based on its sensitivity to δ antisense DNA, is synonymous to the cloned δ opioid receptor. Viewed collectively, these data suggest that administration of δ antisense DNA, and by extension other receptor-selective antisense DNA, is a powerful approach to distinguishing between postulated receptor subtypes.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalRegulatory Peptides
Volume59
Issue number2
DOIs
StatePublished - Oct 20 1995

Keywords

  • Antisense oligodeoxynucleotide
  • Delta opioid receptor
  • Kappa opioid receptor
  • Ligand binding
  • Opioid receptor

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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