Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer

Elizabeth Kanter, Ross Walker, Sam Marion, Patricia Hoyer, Jennifer K. Barton

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

Ovarian cancer is relatively rare but is the fifth leading cause of death from cancer in women. Little is known about the precursors and early stages of ovarian cancer partially due to the lack of a realistic animal model. A cohesive model that incorporates ovarian cancer induction into a menopausal rodent would be well suited for comprehensive studies of ovarian cancer, and non-destructive imaging would allow carcinogenesis to be followed. Optical Coherence Tomography (OCT) and Light-Induced Fluorescence (LIF) are minimally invasive optical modalities that allow both structural and biochemical changes to be noted. Rat ovaries were exposed to 4-vinylcyclohexene diepoxide (VCD) for 20 days in order to destroy the primordial follicles. Sutures coated with 7,12-dimethylbenz(a)anthracene (DMBA) were implanted in the right ovary, in order to produce epithelial based ovarian cancers. Rats were sacrificed at 1, 3, and 5 months and ovaries were harvested and imaged with a combined OCT/LIF system. Histology was preformed on the harvested ovaries and any pathology determined. OCT was able to visualize follicle loss and DMBA-induced abnormalities. LIF spectra were also different between cycling, follicle deplete, and DMBA-exposed ovaries. Overall this pilot study demonstrated the feasibility of both the animal model and optical imaging.

Original languageEnglish (US)
Article number58610P
Pages (from-to)1-8
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5861
StatePublished - Dec 19 2005
EventOptical Coherence Tomography and Coherence Techniques II - Munich, Germany
Duration: Jun 12 2005Jun 16 2005

Keywords

  • Light induced fluorescence
  • Menopause
  • Optical coherence tomography
  • Ovarian cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer'. Together they form a unique fingerprint.

  • Cite this