Optical coherence tomography imaging of collagenous tissue microstructure

Kristi A. Hansen, Jennifer K Barton, Jeffrey A. Weiss

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The engineering of new biomaterials requires an in-depth understanding of the structure and function of the native tissues. Optical coherence tomography (OCT) is a non-destructive technique that allows the visualization of the microstructure of biological tissues. The aim of this study was to determine if OCT could be used to identify geometric properties of tendons such as crimp pattern. Freshly harvested tendon tissue was imaged with OCT during the application of incremental axial strain. Loads were simultaneously recorded during imaging. Results revealed that birefringent banding perpendicular to the collagen fiber axis could be visualized and measured. This crimp banding disappeared at a very low strain. Birefringent banding parallel to the collagen fiber axis was also seen and it was observed that the number of parallel bands increased as strain increased. Stress-strain data was calculated and found to lie within the expected range. These results indicate that OCT may prove to be a useful tool for the non-destructive analysis of tissue microstructure.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages581-587
Number of pages7
Volume3914
Publication statusPublished - 2000
EventLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA
Duration: Jan 22 2000Jan 27 2000

Other

OtherLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
CitySan Jose, CA, USA
Period1/22/001/27/00

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Hansen, K. A., Barton, J. K., & Weiss, J. A. (2000). Optical coherence tomography imaging of collagenous tissue microstructure. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3914, pp. 581-587). Society of Photo-Optical Instrumentation Engineers.