Optical coherence tomography imaging of colonic crypts in a mouse model of colorectal cancer

Weston A. Welge, Jennifer K Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Aberrant crypt foci (ACF) are abnormal epithelial lesions that precede development of colonic polyps. As the earliest morphological change in the development of colorectal cancer, ACF is a highly studied phenomenon. The most common method of imaging ACF is chromoendoscopy using methylene blue as a contrast agent. Narrow- band imaging is a contrast-agent-free modality for imaging the colonic crypts. Optical coherence tomography (OCT) is an attractive alternative to chromoendoscopy and narrow-band imaging because it can resolve the crypt structure at sufficiently high sampling while simultaneously providing depth-resolved data. We imaged in vivo the distal 15 mm of colon in the azoxymethane (AOM) mouse model of colorectal cancer using a commercial swept-source OCT system and a miniature endoscope designed and built in-house. We present en face images of the colonic crypts and demonstrate that different patterns in healthy and adenoma tissue can be seen. These patterns correspond to those reported in the literature. We have previously demonstrated early detection of colon adenoma using OCT by detecting minute thickening of the mucosa. By combining mucosal thickness measurement with imaging of the crypt structure, OCT can be used to correlate ACF and adenoma development in space and time. These results suggest that OCT may be a superior imaging modality for studying the connection between ACF and colorectal cancer.

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
PublisherSPIE
Volume9691
ISBN (Electronic)9781628419252
DOIs
StatePublished - 2016
EventEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III - San Francisco, United States
Duration: Feb 14 2016Feb 15 2016

Other

OtherEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
CountryUnited States
CitySan Francisco
Period2/14/162/15/16

Keywords

  • aberrant crypt foci
  • adenoma
  • colonic crypts
  • colorectal cancer
  • endoscopy
  • optical coherence tomography

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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    Welge, W. A., & Barton, J. K. (2016). Optical coherence tomography imaging of colonic crypts in a mouse model of colorectal cancer. In Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III (Vol. 9691). [96910V] SPIE. https://doi.org/10.1117/12.2211963