Optically transparent recombinant silk-elastinlike protein polymer films

Weibing Teng, Yiding Huang, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Recombinant protein polymers, evaluated exten-sively as biomaterials for applications in drug delivery and tissue engineering, are rarely reported as being optically transparent. Here we report the notable optical transparency of films composed of a genetically engineered silk-elastinlike protein polymer SELP-47K. SELP-47K films of 100 μm in thickness display a transmittance of 93% in the wavelength range of 350-800 nm. While covalent cross-linking of SELP-47K via glutaraldehyde decreases its transmittance to 77% at the wavelength of 800 nm, noncovalent cross-linking using methanol slightly increases it to 95%. Non- and covalent cross-linking of SELP-47K films also influences their secondary structures and water contents. Cell viability and proliferation analyses further reveal the excellent cytocompatibility of both non- and covalently cross-linked SELP-47K films. The combination of high optical transparency and cytocompatibility of SELP-47K films, together with their previously reported outstanding mechanical properties, suggests that this protein polymer may be useful in unique, new biomedical applications.

Original languageEnglish (US)
Pages (from-to)1608-1615
Number of pages8
JournalJournal of Physical Chemistry B
Volume115
Issue number7
DOIs
StatePublished - Feb 24 2011

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silk
Silk
Polymer films
proteins
Proteins
polymers
Polymers
Transparency
transmittance
Recombinant proteins
Wavelength
tissue engineering
Drug delivery
Tissue engineering
viability
Biomaterials
wavelengths
Water content
moisture content
Glutaral

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Optically transparent recombinant silk-elastinlike protein polymer films. / Teng, Weibing; Huang, Yiding; Cappello, Joseph; Wu, Xiaoyi.

In: Journal of Physical Chemistry B, Vol. 115, No. 7, 24.02.2011, p. 1608-1615.

Research output: Contribution to journalArticle

Teng, Weibing ; Huang, Yiding ; Cappello, Joseph ; Wu, Xiaoyi. / Optically transparent recombinant silk-elastinlike protein polymer films. In: Journal of Physical Chemistry B. 2011 ; Vol. 115, No. 7. pp. 1608-1615.
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