Abstract
Fluorescence spectroscopy has shown promise for the in vivo, real-time detection of cervical neoplasia. However, selection of excitation wavelength has in the past been based on in vitro studies and the availability of light sources. The goal of this study was to determine optimal excitation wavelengths for in vivo detection of cervical neoplasia. Fluorescence excitation-emission matrices (EEMs) were measured in vivo from 351 sites in 146 patients. Data were analyzed in pairs of diagnostic classes to determine which combination of excitation wavelengths yields classification algorithms with the greatest sensitivity and specificity. We find that 330-340-, 350-380-, and 400-450-nm excitation yield the best performance. The sensitivity and specificity for discrimination of squamous normal tissue and high-grade squamous intraepithelial lesion (HGSIL) were 71% and 77% on cross validation using three excitation wavelengths. These results are comparable with those found in earlier in vivo studies; however, in this study we find that the proportion of samples which are HGSIL influences performance. Furthermore stratification of samples within low-grade squamous intraepithelial lesion and HGSIL also appears to influence diagnostic performance. Future diagnostic studies should be carried out at these excitation wavelengths in larger groups so that data can be stratified by diagnostic subcategory, age and menopausal status. Similarly, large studies should be done in screening populations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1102-1111 |
| Number of pages | 10 |
| Journal | IEEE Transactions on Biomedical Engineering |
| Volume | 49 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2002 |
Fingerprint
Keywords
- Algorithm
- Cancer diagnosis
- Fluorescence spectroscopy
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Optimal excitation wavelengths for discrimination of cervical neoplasia. / Chang, Sung K.; Follen, Michele; Malpica, Anais; Utzinger, Urs; Staerkel, Gregg; Cox, Dennis; Atkinson, E. Neely; MacAulay, Calum; Richards-Kortum, Rebecca.
In: IEEE Transactions on Biomedical Engineering, Vol. 49, No. 10, 01.10.2002, p. 1102-1111.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimal excitation wavelengths for discrimination of cervical neoplasia
AU - Chang, Sung K.
AU - Follen, Michele
AU - Malpica, Anais
AU - Utzinger, Urs
AU - Staerkel, Gregg
AU - Cox, Dennis
AU - Atkinson, E. Neely
AU - MacAulay, Calum
AU - Richards-Kortum, Rebecca
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Fluorescence spectroscopy has shown promise for the in vivo, real-time detection of cervical neoplasia. However, selection of excitation wavelength has in the past been based on in vitro studies and the availability of light sources. The goal of this study was to determine optimal excitation wavelengths for in vivo detection of cervical neoplasia. Fluorescence excitation-emission matrices (EEMs) were measured in vivo from 351 sites in 146 patients. Data were analyzed in pairs of diagnostic classes to determine which combination of excitation wavelengths yields classification algorithms with the greatest sensitivity and specificity. We find that 330-340-, 350-380-, and 400-450-nm excitation yield the best performance. The sensitivity and specificity for discrimination of squamous normal tissue and high-grade squamous intraepithelial lesion (HGSIL) were 71% and 77% on cross validation using three excitation wavelengths. These results are comparable with those found in earlier in vivo studies; however, in this study we find that the proportion of samples which are HGSIL influences performance. Furthermore stratification of samples within low-grade squamous intraepithelial lesion and HGSIL also appears to influence diagnostic performance. Future diagnostic studies should be carried out at these excitation wavelengths in larger groups so that data can be stratified by diagnostic subcategory, age and menopausal status. Similarly, large studies should be done in screening populations.
AB - Fluorescence spectroscopy has shown promise for the in vivo, real-time detection of cervical neoplasia. However, selection of excitation wavelength has in the past been based on in vitro studies and the availability of light sources. The goal of this study was to determine optimal excitation wavelengths for in vivo detection of cervical neoplasia. Fluorescence excitation-emission matrices (EEMs) were measured in vivo from 351 sites in 146 patients. Data were analyzed in pairs of diagnostic classes to determine which combination of excitation wavelengths yields classification algorithms with the greatest sensitivity and specificity. We find that 330-340-, 350-380-, and 400-450-nm excitation yield the best performance. The sensitivity and specificity for discrimination of squamous normal tissue and high-grade squamous intraepithelial lesion (HGSIL) were 71% and 77% on cross validation using three excitation wavelengths. These results are comparable with those found in earlier in vivo studies; however, in this study we find that the proportion of samples which are HGSIL influences performance. Furthermore stratification of samples within low-grade squamous intraepithelial lesion and HGSIL also appears to influence diagnostic performance. Future diagnostic studies should be carried out at these excitation wavelengths in larger groups so that data can be stratified by diagnostic subcategory, age and menopausal status. Similarly, large studies should be done in screening populations.
KW - Algorithm
KW - Cancer diagnosis
KW - Fluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=0036786044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036786044&partnerID=8YFLogxK
U2 - 10.1109/TBME.2002.803597
DO - 10.1109/TBME.2002.803597
M3 - Article
C2 - 12374334
AN - SCOPUS:0036786044
VL - 49
SP - 1102
EP - 1111
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
SN - 0018-9294
IS - 10
ER -