Optimal experimental design in an epidermal growth factor receptor signalling and down-regulation model

F. P. Casey, D. Baird, Q. Feng, R. N. Gutenkunst, J. J. Waterfall, C. R. Myers, K. S. Brown, R. A. Cerione, J. P. Sethna

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

We apply the methods of optimal experimental design to a differential equation model for epidermal growth factor receptor signalling, trafficking and down-regulation. The model incorporates the role of a recently discovered protein complex made up of the E3 ubiquitin ligase, Cbl, the guanine exchange factor (GEF), Cool-1 (β-Pix) and the Rho family G protein Cdc42. The complex has been suggested to be important in disrupting receptor down-regulation. We demonstrate that the model interactions can accurately reproduce the experimental observations, that they can be used to make predictions with accompanying uncertainties, and that we can apply ideas of optimal experimental design to suggest new experiments that reduce the uncertainty on unmeasurable components of the system.

Original languageEnglish (US)
Pages (from-to)190-202
Number of pages13
JournalIET Systems Biology
Volume1
Issue number3
DOIs
StatePublished - May 1 2007

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ASJC Scopus subject areas

  • Biotechnology
  • Modeling and Simulation
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Casey, F. P., Baird, D., Feng, Q., Gutenkunst, R. N., Waterfall, J. J., Myers, C. R., Brown, K. S., Cerione, R. A., & Sethna, J. P. (2007). Optimal experimental design in an epidermal growth factor receptor signalling and down-regulation model. IET Systems Biology, 1(3), 190-202. https://doi.org/10.1049/iet-syb:20060065