Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: Results from an animal model

Lezlee Coghlan, Urs Utzinger, Rebekah Drezek, Douglas Heintzelman, Andres Zuluaga, Carrie Brookner, Rebecca Richards-Kortum, Irma Gimenez-Conti, Michele Follen

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Using the hamster cheek pouch carcinogenesis model, we explore which fluorescence excitation wavelengths are useful for the detection of neoplasia. 42 hamsters were treated with DMBA to induce carcinogenesis, and 20 control animals were treated only with mineral oil. Fluorescence excitation emission matrices were measured from the cheek pouches of the hamsters weekly. Results showed increased fluorescence near 350-370 nm and 410 nm excitation and decreased fluorescence near 450-470 nm excitation with neoplasia. The optimal diagnostic excitation wavelengths identified using this model - 350-370 nm excitation and 400-450 nm excitation - are similar to those identified for detection of human oral cavity neoplasia.

Original languageEnglish (US)
Pages (from-to)436-446
Number of pages11
JournalOptics Express
Volume7
Issue number12
DOIs
StatePublished - Dec 2000
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Coghlan, L., Utzinger, U., Drezek, R., Heintzelman, D., Zuluaga, A., Brookner, C., Richards-Kortum, R., Gimenez-Conti, I., & Follen, M. (2000). Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: Results from an animal model. Optics Express, 7(12), 436-446. https://doi.org/10.1364/OE.7.000436