Optimum growth conditions and pH control solution for PHB biosynthesis in A. eutrophus

Jeong Kwi Seo, Jeong Yeol Yoon, Joon Taek Oh, Woo Sik Kim

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Optimum growth conditions and pH control solution were obtained in the biosynthesis of PHB(poly-β-hydroxybutyrate) with Alcaligenes eutrophus. Optimum carbon and nitrogen sources were fructose and (NH4) 2SO4, respectively, and optimum additive was 1:1 mixture of yeast extract and polypeptone. To select the optimum pH control solution, various pH control solutions (NaOH, KOH, Na2CO3, and NaOH+KOH) were tested. Na2CO3 solution was found to be the best out of the four solutions tested, because CO2 generated from Na2CO3 could be used as a carbon source. Mixture of NaOH and KOH showed better results than the solution of NaOH or KOH alone. This could be due to the balanced amount of cations (Na+ and K+), which might promote not only the permeation of substrates, but also pumping out Na+ ions.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume4
Issue number3
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • A. eutrophus
  • PHB
  • pH control solution

ASJC Scopus subject areas

  • Chemical Engineering(all)

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