"Orange" You Glad You Checked the Buffy Coat?

Joseph D. Cooper, Dan Dometita, Anjum Hasan, Patrick Dorion, Donna Wolk, Raquel M. Martinez

Research output: Contribution to journalArticle

Abstract

The incidence of tick-borne human infectious syndromes has dramatically risen in the last 2 decades, leading to significant morbidity and mortality. The importance of a rapid and accurate diagnosis to distinguish anaplasmosis from other febrile illnesses is paramount for the prompt administration of appropriate antibiotics and is imperative for the successful treatment of the tick-borne infections. We summarize the current literature describing the biology, clinical presentation, and epidemiology of human granulocytic anaplasmosis. In addition, we report a case of human granulocytic anaplasmosis in one of the less common geographical areas (Pennsylvania) during the winter month of December. Laboratory methods, including use of an acridine orange buffy coat fluorescent stain that helped decreased the time to result and increased confidence in the diagnosis, are also described.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalClinical Microbiology Newsletter
Volume37
Issue number2
DOIs
StatePublished - Jan 15 2015
Externally publishedYes

Fingerprint

Anaplasmosis
Tick-Borne Diseases
Acridine Orange
Ticks
Epidemiology
Fever
Coloring Agents
Anti-Bacterial Agents
Morbidity
Mortality
Incidence
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Cooper, J. D., Dometita, D., Hasan, A., Dorion, P., Wolk, D., & Martinez, R. M. (2015). "Orange" You Glad You Checked the Buffy Coat? Clinical Microbiology Newsletter, 37(2), 9-13. https://doi.org/10.1016/j.clinmicnews.2015.01.001

"Orange" You Glad You Checked the Buffy Coat? / Cooper, Joseph D.; Dometita, Dan; Hasan, Anjum; Dorion, Patrick; Wolk, Donna; Martinez, Raquel M.

In: Clinical Microbiology Newsletter, Vol. 37, No. 2, 15.01.2015, p. 9-13.

Research output: Contribution to journalArticle

Cooper, JD, Dometita, D, Hasan, A, Dorion, P, Wolk, D & Martinez, RM 2015, '"Orange" You Glad You Checked the Buffy Coat?', Clinical Microbiology Newsletter, vol. 37, no. 2, pp. 9-13. https://doi.org/10.1016/j.clinmicnews.2015.01.001
Cooper, Joseph D. ; Dometita, Dan ; Hasan, Anjum ; Dorion, Patrick ; Wolk, Donna ; Martinez, Raquel M. / "Orange" You Glad You Checked the Buffy Coat?. In: Clinical Microbiology Newsletter. 2015 ; Vol. 37, No. 2. pp. 9-13.
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