Organ culture of rat myocardial slices: An alternative in vitro tool in organ-specific toxicology

A. R. Parrish, N. G. Shipp, R. D. Spall, R. T. Dorr, C. L. Krumdieck, A. J. Gandolfi, K. Brendel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Summary Precision-cut adult rat myocardial slices of highly consistent dimensions were produced and maintained for at least 24 h in an organ culture system. These relatively thin (350-400 pm) slices were generated from tissue cores (7 mm diameter) obtained from coring the sagittally slabbed myocardium. The tissue cores were then used to produce slices with a mechanical tissue slicer under specific temperature (25°C) and buffer (zero Ca2+, high K+) conditions. Myocardial slices are viable for at least 24 h in culture, as assessed by a biochemical battery including K+ and Ca2+ content, labeled leucine incorporation, ATP content, and the loss of the cytoplasmic enzyme LDH. In addition to these parameters, histological evaluation was also employed. These data indicate that the precision-cut myocardial slice system is a possible alternative in vitro tool and merits further study in the field of cardiotoxicity.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalToxicology Mechanisms and Methods
Volume2
Issue number2
DOIs
StatePublished - Jan 1 1992

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Keywords

  • Methodology
  • Rat myocardial slices

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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