Organ culture of rat myocardial slices

An alternative in vitro tool in organ-specific toxicology

A. R. Parrish, N. G. Shipp, R. D. Spall, Robert T Dorr, C. L. Krumdieck, A Jay Gandolfi, K. Brendel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Summary Precision-cut adult rat myocardial slices of highly consistent dimensions were produced and maintained for at least 24 h in an organ culture system. These relatively thin (350-400 pm) slices were generated from tissue cores (7 mm diameter) obtained from coring the sagittally slabbed myocardium. The tissue cores were then used to produce slices with a mechanical tissue slicer under specific temperature (25°C) and buffer (zero Ca2+, high K+) conditions. Myocardial slices are viable for at least 24 h in culture, as assessed by a biochemical battery including K+ and Ca2+ content, labeled leucine incorporation, ATP content, and the loss of the cytoplasmic enzyme LDH. In addition to these parameters, histological evaluation was also employed. These data indicate that the precision-cut myocardial slice system is a possible alternative in vitro tool and merits further study in the field of cardiotoxicity.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalToxicology Mechanisms and Methods
Volume2
Issue number2
DOIs
StatePublished - 1992

Fingerprint

Organ Culture Techniques
Toxicology
Rats
Tissue
Leucine
Myocardium
Buffers
Adenosine Triphosphate
Temperature
Enzymes
In Vitro Techniques

Keywords

  • Methodology
  • Rat myocardial slices

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Organ culture of rat myocardial slices : An alternative in vitro tool in organ-specific toxicology. / Parrish, A. R.; Shipp, N. G.; Spall, R. D.; Dorr, Robert T; Krumdieck, C. L.; Gandolfi, A Jay; Brendel, K.

In: Toxicology Mechanisms and Methods, Vol. 2, No. 2, 1992, p. 101-111.

Research output: Contribution to journalArticle

Parrish, A. R. ; Shipp, N. G. ; Spall, R. D. ; Dorr, Robert T ; Krumdieck, C. L. ; Gandolfi, A Jay ; Brendel, K. / Organ culture of rat myocardial slices : An alternative in vitro tool in organ-specific toxicology. In: Toxicology Mechanisms and Methods. 1992 ; Vol. 2, No. 2. pp. 101-111.
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