Organization of actin cytoskeleton during early endothelial regeneration in vitro

G. Gabbiani, F. Gabbiani, Ronald L Heimark, S. M. Schwartz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The pattern of early cell movement after an experimental 'wound' and the organization of actin in stationary and moving cultured endothelial cells have been studied by means of: time-lapse photography; indirect immunofluorescence using anti-actin antibodies with and without pretreatment with the actin destabilizing factor present in human plasma; and differential centrifugation and densitometric analysis of stained sodium dodecylsulphate/polyacrylamide gels in order to evaluate the total and relative amounts of G and F-actin. Up to 5 h after a single scratch, movement consists of a coordinate spreading and translocation of a band of about 10 cells from the wound edge. Compared to stationary cells, moving endothelial cells show: no significant changes in the intensity and distribution of immunofluorescent staining with anti-actin antibodies, but an increased sensitivity of cytoplasmic actin, including stress fibres, to the actin-destabilizing factor purified from human plasma; and no significant change in the total amount of actin, but a decreased relative amount of F-actin and a corresponding increased relative amount of G-actin. We conclude that endothelial cell movement in vitro is accompanied by a rapid change in the state of actin organization characterized by an overall decrease in cytoplasmic F-actin.

Original languageEnglish (US)
Pages (from-to)39-50
Number of pages12
JournalJournal of Cell Science
VolumeVOL. 66
StatePublished - 1984
Externally publishedYes

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Actin Cytoskeleton
Actins
Regeneration
Endothelial Cells
Cell Movement
In Vitro Techniques
Anti-Idiotypic Antibodies
Stress Fibers
Photography
Wounds and Injuries
Indirect Fluorescent Antibody Technique
Centrifugation
Cultured Cells
Sodium
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology

Cite this

Organization of actin cytoskeleton during early endothelial regeneration in vitro. / Gabbiani, G.; Gabbiani, F.; Heimark, Ronald L; Schwartz, S. M.

In: Journal of Cell Science, Vol. VOL. 66, 1984, p. 39-50.

Research output: Contribution to journalArticle

Gabbiani, G. ; Gabbiani, F. ; Heimark, Ronald L ; Schwartz, S. M. / Organization of actin cytoskeleton during early endothelial regeneration in vitro. In: Journal of Cell Science. 1984 ; Vol. VOL. 66. pp. 39-50.
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