Organotypic 3D cell culture models: Using the rotating wall vessel to study hostĝ€"pathogen interactions

Jennifer Barrila, Andrea L. Radtke, Auŕlie Crabbé, Shameema F. Sarker, Melissa M. Herbst-Kralovetz, C. Mark Ott, Cheryl A. Nickerson

Research output: Contribution to journalReview article

155 Scopus citations


Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of hostĝ€" pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

Original languageEnglish (US)
Pages (from-to)791-801
Number of pages11
JournalNature Reviews Microbiology
Issue number11
StatePublished - Nov 1 2010


ASJC Scopus subject areas

  • Microbiology
  • Immunology and Microbiology(all)
  • Infectious Diseases

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