Ortholog alleles at Xa3/Xa26 locus confer conserved race-specific resistance against xanthomonas oryzae in rice

Hong Jing Li, Xiang Hua Li, Jing Hua Xiao, Rod A. Wing, Shi Ping Wang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The rice disease resistance (R) gene Xa3/Xa26 (having also been named Xa3 and Xa26) against Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight disease, belongs to a multiple gene family clustered in chromosome 11 and is from an AA genome rice cultivar (Oryza sativa L.). This family encodes leucine-rich repeat (LRR) receptor kinase-type proteins. Here, we show that the orthologs (alleles) of Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3, from wild Oryza species O. officinalis (CC genome) and O. minuta (BBCC genome), respectively, were also R genes against Xoo. Xa3/Xa26-2 and Xa3/Xa26-3 conferred resistance to 16 of the 18 Xoo strains examined. Comparative sequence analysis of the Xa3/Xa26 families in the two wild Oryza species showed that Xa3/Xa26-3 appeared to have originated from the CC genome of O. minuta. The predicted proteins encoded by Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3 share 91-99% sequence identity and 94-99% sequence similarity. Transgenic plants carrying a single copy of Xa3/Xa26, Xa3/Xa26-2, or Xa3/Xa26-3, in the same genetic background, showed a similar resistance spectrum to a set of Xoo strains, although plants carrying Xa3/Xa26-2 or Xa3/Xa26-3 showed lower resistance levels than the plants carrying Xa3/Xa26. These results suggest that the Xa3/Xa26 locus predates the speciation of A and C genome, which is approximately 7.5million years ago. Thus, the resistance specificity of this locus has been conserved for a long time.

Original languageEnglish (US)
Pages (from-to)281-290
Number of pages10
JournalMolecular Plant
Volume5
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Bacterial blight
  • Oryza minuta
  • Oryza officinalis
  • Oryza sativa
  • broad-spectrum resistance
  • durable resistance

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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