Osteopontin regulates α-smooth muscle actin and calponin in vascular smooth muscle cells

Hong Gao, Marlene C. Steffen, Kenneth Ramos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

vSMCs (vascular smooth muscle cells) lose differentiation markers and gain uncontrolled proliferative activity during the early stages of atherosclerosis. Previous studies have shown that OPN (osteopontin) mRNA and protein levels increase significantly on induction of proliferative activity by allylamine (an atherogenic amine) and that this response can be inhibited by OPN antibodies. We have investigated the role of OPN in vSMC differentiation. Primary cultures of aortic mouse vSMCs were transfected with an OPN expression plasmid and several vSMC differentiation markers including α-SM actin (a-smooth muscle actin), SM22-α, tropomyosin and calponin were monitored in this cellular model. α-SM actin and calponin protein levels were significantly decreased by OPN overexpression. Down-regulation of α-SM actin and calponin was also observed on extracellular treatment of mouse vSMCs with recombinant OPN. In addition, calponin mRNA was significantly decreased under serum-restricted conditions when OPN mRNA was dramatically increased, while α-SM actin mRNA remained unchanged. These data indicate that OPN down-regulates a-SM actin and calponin expression through an extracellular signalling pathway. Functional connectivity between OPN and vSMC differentiation markers has been established. Since vSMCs lose differentiation features during early atherosclerosis, a mechanistic basis for OPN functions as a critical regulator of proliferative cardiovascular disease has been presented.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalCell Biology International
Volume36
Issue number2
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

Fingerprint

Osteopontin
Vascular Smooth Muscle
Smooth Muscle Myocytes
Smooth Muscle
Actins
Cell Differentiation
Differentiation Antigens
Messenger RNA
Atherosclerosis
Down-Regulation
Allylamine
calponin
Tropomyosin
Amines
Proteins
Plasmids
Cardiovascular Diseases

Keywords

  • Actin
  • Calponin
  • Osteopontin
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Osteopontin regulates α-smooth muscle actin and calponin in vascular smooth muscle cells. / Gao, Hong; Steffen, Marlene C.; Ramos, Kenneth.

In: Cell Biology International, Vol. 36, No. 2, 01.02.2012, p. 155-161.

Research output: Contribution to journalArticle

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