Overexpression of 14-3-3γ causes polyploidization in H322 lung cancer cells

Wenqing Qi, Xiaobing Liu, Weiming Chen, Qiang Li, Jesse D Martinez

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The 14-3-3 proteins are a family of highly conserved proteins that participate in a wide variety of cellular processes. Mounting evidence suggests that 14-3-3 proteins have a role in human cancers, however their role in tumorigenesis is unclear. Here we report that over-expression of 14-3-3 γ protein in human lung cancer cell line H322 results in abnormal DNA replication and polyploidization. Cells that overexpress 14-3-3 γ are resistant to microtubule inhibitors and can reenter the cell cycle in the absence of mitosis suggesting that elevated levels of 14-3-3 γ may enable cells to bypass the mitotic checkpoint. Taken together, our data indicate that 14-3-3γ may contribute to tumorigenesis by promoting genomic instability.

Original languageEnglish (US)
Pages (from-to)847-856
Number of pages10
JournalMolecular Carcinogenesis
Volume46
Issue number10
DOIs
StatePublished - Oct 2007

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14-3-3 Proteins
Lung Neoplasms
Carcinogenesis
M Phase Cell Cycle Checkpoints
Genomic Instability
DNA Replication
Mitosis
Microtubules
Cell Cycle
Cell Line
Neoplasms
Proteins

Keywords

  • 14-3-3
  • Cell cycle
  • Lung cancer
  • Polyploidization

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Overexpression of 14-3-3γ causes polyploidization in H322 lung cancer cells. / Qi, Wenqing; Liu, Xiaobing; Chen, Weiming; Li, Qiang; Martinez, Jesse D.

In: Molecular Carcinogenesis, Vol. 46, No. 10, 10.2007, p. 847-856.

Research output: Contribution to journalArticle

Qi, Wenqing ; Liu, Xiaobing ; Chen, Weiming ; Li, Qiang ; Martinez, Jesse D. / Overexpression of 14-3-3γ causes polyploidization in H322 lung cancer cells. In: Molecular Carcinogenesis. 2007 ; Vol. 46, No. 10. pp. 847-856.
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